高效液相色谱法测定压片糖果和固体饮料中2种新型卡巴地那非类似物
目的 建立高效液相色谱法测定压片糖果和固体饮料中2种新型卡巴地那非类似物的方法.方法 样品经甲醇超声提取,提取液离心、过微孔滤膜后上机分析.采用Waters Xbridge C18色谱柱(250 mm×4.6 mm,5 μm)分离,以20 mmol/L乙酸铵水溶液和甲醇为流动相进行梯度洗脱,外标法定量测定.结果 在0.5～100.0 μg/mL的范围内2种新型卡巴地那非类似物线性关系良好(r2＞0.999),定量限均为5 mg/kg.2种新型卡巴地那非类似物于1、2、10倍定量限3个水平下的平均回收率范围为87.32％～93.92％,相对标准偏差为1.19％～4.08％.采用该方法共检出8批次...
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| Published in | 食品安全质量检测学报 Vol. 16; no. 3; pp. 235 - 240 |
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| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
湖北省食品质量安全监督检验研究院,武汉 430075
2025
国家市场监督管理总局重点实验(动物源性食品中重点化学危害物检测技术),武汉 430075 湖北时珍实验室,武汉 430065%沃特世科技(上海)有限公司,上海 201206 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 2095-0381 |
| DOI | 10.19812/j.cnki.jfsq11-5956/ts.20240828002 |
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| Abstract | 目的 建立高效液相色谱法测定压片糖果和固体饮料中2种新型卡巴地那非类似物的方法.方法 样品经甲醇超声提取,提取液离心、过微孔滤膜后上机分析.采用Waters Xbridge C18色谱柱(250 mm×4.6 mm,5 μm)分离,以20 mmol/L乙酸铵水溶液和甲醇为流动相进行梯度洗脱,外标法定量测定.结果 在0.5～100.0 μg/mL的范围内2种新型卡巴地那非类似物线性关系良好(r2＞0.999),定量限均为5 mg/kg.2种新型卡巴地那非类似物于1、2、10倍定量限3个水平下的平均回收率范围为87.32％～93.92％,相对标准偏差为1.19％～4.08％.采用该方法共检出8批次阳性样品,含量范围为218～2170mg/kg.结论 该方法操作简便,准确可靠,满足压片糖果和固体饮料中非法添加2种新型卡巴地那非类似物的含量测定,为打击食品中非法添加新型那非类物质提供可行的技术手段. |
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| AbstractList | 目的 建立高效液相色谱法测定压片糖果和固体饮料中2种新型卡巴地那非类似物的方法.方法 样品经甲醇超声提取,提取液离心、过微孔滤膜后上机分析.采用Waters Xbridge C18色谱柱(250 mm×4.6 mm,5 μm)分离,以20 mmol/L乙酸铵水溶液和甲醇为流动相进行梯度洗脱,外标法定量测定.结果 在0.5～100.0 μg/mL的范围内2种新型卡巴地那非类似物线性关系良好(r2＞0.999),定量限均为5 mg/kg.2种新型卡巴地那非类似物于1、2、10倍定量限3个水平下的平均回收率范围为87.32％～93.92％,相对标准偏差为1.19％～4.08％.采用该方法共检出8批次阳性样品,含量范围为218～2170mg/kg.结论 该方法操作简便,准确可靠,满足压片糖果和固体饮料中非法添加2种新型卡巴地那非类似物的含量测定,为打击食品中非法添加新型那非类物质提供可行的技术手段. |
| Abstract_FL | Objective To establish a method for determination of 2 kinds of new carbadenafil analogue in tablet candies and solid beverages by high performance liquid chromatography.Methods The sample was extracted by methanol ultrasound,the extract was centrifuged,filtered through a microporous membrane,and analyzed by the high performance liquid chromatography.The separation was performed on a Waters Xbridge C18 column(250 mmx4.6 mm,5 μm)with gradient elution by using 20 mmol/L ammonium acetate aqueous solution and methanol as the mobile phase,and quantified by external standard method.Results The 2 kinds of new carbadenafil analogue demonstrated good linearity in the range of 0.5-100.0 μg/mL,with the correlation coefficient values(r2)being higher than 0.999.The limits of quantification were found to be 5 mg/kg.The recoveries at 3 spiked levels of 1,2 and 10 times the limits of quantification in blank matrix were in the range of 87.32％-93.92％,with the relative standard deviations between 1.19％-4.08％.A total of 8 batches of positive samples were detected using this method,with a content range of 218-2170 mg/kg.Conclusion The method is convenient,accurate and efficient.It can meet the identification requirement of illegally added 2 kinds of new carbadenafil analogue in tablet candies and solid beverages,and provide effective technical support to combat the illegal addition of new carbadenafil analogue in food. |
| Author | 朱松松 韩智 张莉 冯猛 吴婉琴 夏金涛 范小龙 江丰 张亚珍 |
| AuthorAffiliation | 湖北省食品质量安全监督检验研究院,武汉 430075;国家市场监督管理总局重点实验(动物源性食品中重点化学危害物检测技术),武汉 430075;湖北时珍实验室,武汉 430065%沃特世科技(上海)有限公司,上海 201206 |
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| Author_FL | WU Wan-Qin JIANG Feng ZHANG Li FENG Meng XIA Jin-Tao ZHANG Ya-Zhen HAN Zhi ZHU Song-Song FAN Xiao-Long |
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| Keywords | 固体饮料 high performance liquid chromatography 压片糖果 tablet candies illegal additions solid beverages 高效液相色谱法 非法添加 carbadenafil analogue 卡巴地那非类似物 |
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| PublicationTitle | 食品安全质量检测学报 |
| PublicationTitle_FL | Journal of Food Safety & Quality |
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| Publisher | 湖北省食品质量安全监督检验研究院,武汉 430075 国家市场监督管理总局重点实验(动物源性食品中重点化学危害物检测技术),武汉 430075 湖北时珍实验室,武汉 430065%沃特世科技(上海)有限公司,上海 201206 |
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| Snippet | 目的 建立高效液相色谱法测定压片糖果和固体饮料中2种新型卡巴地那非类似物的方法.方法 样品经甲醇超声提取,提取液离心、过微孔滤膜后上机分析.采用Waters Xbridge C18色谱柱(250 mm×4.6 mm,5 μm)分离,以20 mmol/L乙酸铵水溶液和甲醇为流动相进行梯度洗脱,外标法定量测定.结果... |
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| Title | 高效液相色谱法测定压片糖果和固体饮料中2种新型卡巴地那非类似物 |
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