A bilayered nanoshell for durable protection of single yeast cells against multiple, simultaneous hostile stimuliElectronic supplementary information (ESI) available. See DOI: 10.1039/c8sc01130c
Single cell surface engineering provides the most efficient, non-genetic strategy to enhance cell stability. However, it remains a huge challenge to improve cell stability in complex artificial environments. Here, a soft biohybrid interfacial layer is fabricated on individual living-cell surfaces by...
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| Main Authors | , , , , , , , , |
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| Format | Journal Article |
| Published |
30.05.2018
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| Online Access | Get full text |
| ISSN | 2041-6520 2041-6539 |
| DOI | 10.1039/c8sc01130c |
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| Abstract | Single cell surface engineering provides the most efficient, non-genetic strategy to enhance cell stability. However, it remains a huge challenge to improve cell stability in complex artificial environments. Here, a soft biohybrid interfacial layer is fabricated on individual living-cell surfaces by their exposure to a suspension of gold nanoparticles and
l
-cysteine to form a protecting functional layer to which porous silica layers were bound yielding pores with a diameter of 3.9 nm. The living cells within the bilayered nanoshells maintained high viability (96 ± 2%) as demonstrated by agar plating, even after five cycles of simultaneous exposure to high temperature (40 °C), lyticase and UV light. Moreover, yeast cells encapsulated in bilayered nanoshells were more recyclable than native cells due to nutrient storage in the shell.
A bilayered nanoshell is used to encapsulate a single cell for high stability after hostile stimulation. |
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| AbstractList | Single cell surface engineering provides the most efficient, non-genetic strategy to enhance cell stability. However, it remains a huge challenge to improve cell stability in complex artificial environments. Here, a soft biohybrid interfacial layer is fabricated on individual living-cell surfaces by their exposure to a suspension of gold nanoparticles and
l
-cysteine to form a protecting functional layer to which porous silica layers were bound yielding pores with a diameter of 3.9 nm. The living cells within the bilayered nanoshells maintained high viability (96 ± 2%) as demonstrated by agar plating, even after five cycles of simultaneous exposure to high temperature (40 °C), lyticase and UV light. Moreover, yeast cells encapsulated in bilayered nanoshells were more recyclable than native cells due to nutrient storage in the shell.
A bilayered nanoshell is used to encapsulate a single cell for high stability after hostile stimulation. |
| Author | Su, Bao-Lian Ying, Guo-Liang Yetisen, Ali K Busscher, Henk J Shen, Ling Jiang, Nan Yang, Xiao-Yu Montelongo, Yunuen Xiao, Yu-Xuan |
| AuthorAffiliation | University of Birmingham School of Materials Science and Engineering University of Groningen Wuhan University of Technology Brigham and Women's Hospital School of Engineering and Applied Sciences Harvard University Department of Biomedical Engineering University of Namur School of Chemical Engineering Universidad De La Salle Bajío Division of Engineering in Medicine Laboratory of Inorganic Materials Chemistry Harvard Medical School State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan Institute of Technology University Medical Center Groningen |
| AuthorAffiliation_xml | – name: State Key Laboratory of Advanced Technology for Materials Synthesis and Processing – name: Laboratory of Inorganic Materials Chemistry – name: School of Engineering and Applied Sciences – name: School of Materials Science and Engineering – name: Wuhan Institute of Technology – name: University Medical Center Groningen – name: School of Chemical Engineering – name: Wuhan University of Technology – name: Department of Biomedical Engineering – name: Division of Engineering in Medicine – name: Harvard Medical School – name: University of Namur – name: Harvard University – name: Universidad De La Salle Bajío – name: University of Birmingham – name: University of Groningen – name: Brigham and Women's Hospital |
| Author_xml | – sequence: 1 givenname: Nan surname: Jiang fullname: Jiang, Nan – sequence: 2 givenname: Guo-Liang surname: Ying fullname: Ying, Guo-Liang – sequence: 3 givenname: Ali K surname: Yetisen fullname: Yetisen, Ali K – sequence: 4 givenname: Yunuen surname: Montelongo fullname: Montelongo, Yunuen – sequence: 5 givenname: Ling surname: Shen fullname: Shen, Ling – sequence: 6 givenname: Yu-Xuan surname: Xiao fullname: Xiao, Yu-Xuan – sequence: 7 givenname: Henk J surname: Busscher fullname: Busscher, Henk J – sequence: 8 givenname: Xiao-Yu surname: Yang fullname: Yang, Xiao-Yu – sequence: 9 givenname: Bao-Lian surname: Su fullname: Su, Bao-Lian |
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| References_xml | – issn: 2003 publication-title: Yeast as a model genetic organism doi: Mell Burgess – issn: 2005 publication-title: Computational electrodynamics: the finite-difference time-domain method doi: Taflove Hagness – issn: 2014 publication-title: Cytocompatibility and Toxicity of Functional Coatings Engineered at Cell Surfaces doi: Kharlampieva Kozlovskaya |
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| Title | A bilayered nanoshell for durable protection of single yeast cells against multiple, simultaneous hostile stimuliElectronic supplementary information (ESI) available. See DOI: 10.1039/c8sc01130c |
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