A bilayered nanoshell for durable protection of single yeast cells against multiple, simultaneous hostile stimuliElectronic supplementary information (ESI) available. See DOI: 10.1039/c8sc01130c

Single cell surface engineering provides the most efficient, non-genetic strategy to enhance cell stability. However, it remains a huge challenge to improve cell stability in complex artificial environments. Here, a soft biohybrid interfacial layer is fabricated on individual living-cell surfaces by...

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Main Authors Jiang, Nan, Ying, Guo-Liang, Yetisen, Ali K, Montelongo, Yunuen, Shen, Ling, Xiao, Yu-Xuan, Busscher, Henk J, Yang, Xiao-Yu, Su, Bao-Lian
Format Journal Article
Published 30.05.2018
Online AccessGet full text
ISSN2041-6520
2041-6539
DOI10.1039/c8sc01130c

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Summary:Single cell surface engineering provides the most efficient, non-genetic strategy to enhance cell stability. However, it remains a huge challenge to improve cell stability in complex artificial environments. Here, a soft biohybrid interfacial layer is fabricated on individual living-cell surfaces by their exposure to a suspension of gold nanoparticles and l -cysteine to form a protecting functional layer to which porous silica layers were bound yielding pores with a diameter of 3.9 nm. The living cells within the bilayered nanoshells maintained high viability (96 ± 2%) as demonstrated by agar plating, even after five cycles of simultaneous exposure to high temperature (40 °C), lyticase and UV light. Moreover, yeast cells encapsulated in bilayered nanoshells were more recyclable than native cells due to nutrient storage in the shell. A bilayered nanoshell is used to encapsulate a single cell for high stability after hostile stimulation.
Bibliography:10.1039/c8sc01130c
Electronic supplementary information (ESI) available. See DOI
ISSN:2041-6520
2041-6539
DOI:10.1039/c8sc01130c