Voltage sensitive optical mapping used to observe effects of late Na and rapidly activating delayed rectifier K currents on the right and left ventricular electrophysiological heterogeneity / 应用光标测技术观察INa-L和IKr对左、右心室间电异质性的影响

• Published in: Xi'an jiao tong da xue xue bao. Journal of Xi'an Jiaotong University (medical sciences). Yi xue ban no. 4; p. 500
• Main Authors: Hong-mei, SUN, Li, LI, Yong-rong, JIANG, YANG, Lin, Xiao-lin, XUE, KONG, Shu, Zhao, YANG
• Format: Journal Article
• Language: Chinese
• Published: Xi'an Xi'an Jiaotong University 01.01.2016
• ISSN: 1671-8259
• DOI: 10.7652/jdyxb201604007

Objective: To observe the effects of late Na current (INa-L) and rapidly activating delayed rectifier K current (IKr) on ventricular heterogeneity and frequency dependency by using high resolution voltage sensitive optical mapping technology. Methods: The model of 12 isolated hearts was constructed in rabbits. Voltage sensitive dye Di-4-ANEPPS were perfused into the isolated hearts by Langendorff method. LED source with the wave length of 532 nm was used to record APD80 and APD50 of the left and right ventricles. Experimental groups were divided into 3 groups by perfusion drugs dofetillide (30nmol/L), dofetillide+ATX-Ⅱ(1nmol/L), and dofetillide + ATX-Ⅱ+mexiletine (10μmol/L). The subjects were intervened by the above drugs in order, and they were self-compared before dosing. After each drug administration, the hearts were stimulated respectively with the BCL of 2000ms, 1000ms, 500ms, and 300ms. Then we observed the changes of APD80 and APD50 in the left and right ventricles before and after the interventions.