Stability Analysis of Reference Genes for Larch Gene Expression Studies by Quantitative Real-Time PCR / 落叶松实时定量PCR内参基因的筛选

• Published in: Forest research (Beijing) Vol. 26; no. S1; p. 1
• Main Authors: 吴涛, 李万峰, 张俊红, 韩素英, 杨文华, 齐力旺
• Format: Journal Article
• Language: Chinese
• Published: Beijing Chinese Academy of Forestry 01.01.2013
• Subjects: Gene expression
• ISSN: 1001-1498

The selection of suitable reference gene is an important prerequisite for successful gene expression analysis by quantitative real-time PCR (qPCR). The authors investigated the expression stability of 12 endogenous house-keeping genes (ACT4, APm, Chc, Gapc, RPL1, RPL2, EF1, EF2, eIF, E3UL, UBQ and UPL ) in 31 samples of Larix kaempferi (Lamb.) Carr. including needles, stems, roots, pollens, zygotic embryos and somatic embryos. The GeNorm and NormFinder algorithms analysis reveals that the APm is the most stable gene among different organs and developmental stages (somatic embryogenesis and seed germination), EF and eIF are most stable among stems and needles during plant growth (90 days, 1.5 years, 5 years, 10 years, 25 years and 50 year), respectively. The results would provide optimum internal reference genes for larch gene expression analysis using qPCR. 本研究针对日本落叶松(Larix kaempferi (Lamb.) Carr.)体细胞胚胎发育过程中原胚团时期到胚胎成熟时期、种子萌发过程、植株幼年生长阶段和成年生长阶段等生长发育过程中的31份材料,应用实时荧光定量PCR(qPCR)技术,分析了12个持家基因(ACT 4、APm、Chc、Gapc、RPL