D98 INSIGHTS INTO ENVIRONMENTAL EXPOSURES IN ASTHMA, COPD, AND CONSTRICTIVE BRONCHIOLITIS: Rhinovirus Induces Dysmaturity And Unjamming Of Normal And Asthmatic Human Bronchial Epithelial Layers

• Published in: American journal of respiratory and critical care medicine Vol. 195
• Main Authors: Rhodes, C, Mitchel, J, Bochkov, Y A, Hirsch, R, Lan, B, Stancil, I, Gern, J E, Butler, J P, Fredberg, J J, Park, J-A
• Format: Journal Article
• Language: English
• Published: New York American Thoracic Society 01.01.2017
• Subjects: Asthma; Chronic obstructive pulmonary disease; Infections; Pathogenesis; Velocity
• ISSN: 1073-449X; 1535-4970

Rationale Human Rhinovirus (RV) is implicated in asthma pathogenesis and is a known asthma trigger1,2, but mechanism remains unclear.3 The maturing layer of primary human bronchial epithelial cells (HBECs) derived from healthy or asthmatic donors undergoes a transition from a migratory, fluid-like, unjammed phase to non-migratory, solid-like jammed phase. 4 But in the mature jammed HBEC layer, mechanical compression as occurs during bronchospasm provokes dysmature layer unjamming and airway wall remodeling .4 Using the mature jammed HBEC layer, here we test the hypothesis that RV infection, too, can provoke dysmature layer unjamming. Time-lapse images were taken for 24 hrs. starting at 2 hrs. after RV infection on ALI day 14, and Particle Image Velocimetry (PIV) was used to measure cell motility.