Astaxantlhin Production iln Transgenic Arab i lopsis with chyB Gene Encoding ]3-carotene Hydr : , 'lase

• Published in: Journal of plant biology = Singmul Hakhoe chi pp. 58 - 63
• Main Author: 강권규
• Format: Journal Article
• Language: English
• Published: 한국식물학회 01.01.2008
• Subjects: 생물학
• ISSN: 1226-9239; 1867-0725

Oxyca:otenoids, produced through the oxidation of carotenoids, play critical roles in plants. Tlhi.~, reaction is ,mediated by a specific enzyme, ]3-carotene hydroxylase, which adds hydroxyl groups to the ]3-rings of carotenes. To investigate the effec~ of the ~-carotene hydroxylase gene (Chyb) on oxycarotenoid biosynthesis, we generated transgenic Arabidopsi~ plants thai over-expressed Chyb under the control of a 35S promoter. Their levels of zeaxanthin and neoxanthin were two- to tb.ree-fold greater relative to the ~ wh~le llhal~ of violaxanthin, a final product in the xanlthophyll pathway, was 1.3-fold higher than the control. In co~trast, the amount of ~3-carotene declined as much as 2.4-fold, depending on the particular transgenic line. Interesltingly, astaxanthin was produced in the transgenics, but not in the WT. These data suggest that, with the aid of unknown factors in li]e host, carotenoids could be convetted into metabolites in the astaxanthin biosynthetic pathway. Microarray analysis was used Io identify several genes that were consistently up-or down-regulated in transgenic chylB leaves compared with the controls. Here. we also discuss possible modifications in leaf carotenoids, and the importance of these data from a nutritional standpoint. KCI Citation Count: 6