Expression of Protein Kinase C on Allergic Nasal Mucosa

• Published in: Journal of rhinology pp. 38 - 43
• Main Authors: 송영호, 홍남표, 조중생, 최희석, 안회영
• Format: Journal Article
• Language: English
• Published: 대한비과학회 01.05.1998
• Subjects: 이비인후과학
• ISSN: 1229-1498; 2384-4361

Protein Kinase C (PKC) relays information in the form of a various extracellar signals across the membrane and is known toplay an important role in the production of B lymphocytes and T lymphocytes, in the antigen-presentation of Langerhans cell,and in inflammatory reactions. The presentation of allergens to T lymphocytes is likely an important aspect in the pathophysiologicalmechanism of allergic rhinitis. Although several theories have been formulated in allergic rhinitis, signal transduction ofthis disease remains unknown. In this study, we focused on the role of the enzyme PKC in the allergic mucosa of the nose. Specifically, we investigated the role and the distribution of PKC isozymes in the mucous membrane of the nose. We obtainednasal mucous membrane specimens from 10 patients with house dust mite allergy and 10 patients with normal nasal mucousmembrane. We performed an immunohistochemical study, an RT-PCR, and a densitometric measurement. PKCα, PKCβ, andPKCζ proteins were detected in the subepithelial layer of the allergic mucosa. However there were no detectable reactions inthe nonallergic mucosa. In the RT-PCR for PKCα, PKCβ, and PKCζ, there was no difference between the allergic and nonallergicnasal mucous membrane in terms of m-RNA expression. It is possible that the signal transduction pathway of PKC andover-expression of PKC protein at the post-transcription level contribute to the development of allergic inflammation in allergicnasal mucosa. KCI Citation Count: 0