인공 혈관용 나노 섬유 지지체에 관한 연구 : 세포 부착 및 조직 형성

• Published in: Biomaterials research pp. 100 - 108
• Main Authors: 신호준, 이용재, 김인애, 신정욱, 이창훈, 조은희, 김은정
• Format: Journal Article
• Language: Korean
• Published: 한국생체재료학회 01.06.2004
• Subjects: 의공학
• ISSN: 1226-4601; 2055-7124

The purpose of this study is to examine the potential of the nanofiber scaffold as a substrate for forming an artificial vascular graft. Aligned and randomly aligned polylatic-co-glycolic acid (PLGA) and polyurethane(PU) nanofiber scaffolds fabricated by electrospinning were used as square sheets. Fibroblasts harvested from human foreskin were seeded and cultured over 1 week period on a nanofiber scaffold, followed by seeding of endothelial cells harvested from human vessel. Thereafter the new tissue was analyzed for DNA, collagen and stained for immunohistochemistry with von Willebrand factor. DNA and collagen analysis of the seeded scaffold demonstrated that the human fibroblasts and endothelial cells attached to the polymeric fibers had begun to spread out (day 7) and extracellular matrix was saturated after 2 week. The immunohistochemistry examination demonstrated the seeded endothelial cells formed a monolayer on the fibroblasts and no endothelial cell invasion or new formation of capillaries could be detected. Indeed, there's no significant difference between aligned and randomly aligned nanofiber scaffold in all analyses. These results demonstrated that nanofiber scaffold used in this study might be a promising base material for tissue-engineered blood vessels. KCI Citation Count: 0