연골세포의 고분자 지지체 접종시 다중 동적 접종법의 효과

• Published in: Biomaterials research pp. 215 - 222
• Main Authors: 박상혁, 박소라, 민병현, 박태순
• Format: Journal Article
• Language: Korean
• Published: 한국생체재료학회 01.12.2003
• Subjects: 의공학
• ISSN: 1226-4601; 2055-7124

In the field of the cartilage regeneration in vitro, there are several essential factors to maintain the high cell density and even cell distribution on the scaffold. Limited donor cells or uneven cell distribution by high cell density seeding, however, have been unsolved problem. In this study, therefore, multi-seeding method was developed to increase the seeding efficiency and uniform cell distribution in chondrocyte/PGA scaffold. Three consecutive single dynamic seedings were made with the inoculation density of 4´106 cells/cm3. After the 3rd multi-seeding (total; 1.2´107 cells/cm3), the yield maintained above 93% with attached cell numbers 1.12´107 cells. The even cell distribution was confirmed by H&E staining and SEM after the 3rd multi-seeding and also, immunohistochemically tested for the expression of type I and type II collagen comparing to single dynamic seeding. Cells on polymer scaffolds were synthesized the ECM components such as proteoglycan and collagen. However, their synthetic rate was not proportional to the attached cell number showing the peaks at the 1st and 2nd seeding. From these results, multi-seeding method is superior way to attach high density chondrocytes in the PGA scaffold comparing to single cell seeding method. Moreover, even cell distribution was sustained in the multi-seeding method. This method will provide the useful tool for fabrication of articular cartilage KCI Citation Count: 0