Biodegradable Characteristics of Novel Sustained Release Collagen Film Containing 5-Fluorouracil (5-FU) and mitomycin-C (MMC)

• Published in: Biomaterials research pp. 248 - 253
• Main Authors: 김동명, 김태경, 주전기
• Format: Journal Article
• Language: Korean
• Published: 한국생체재료학회 01.12.2003
• Subjects: 의공학
• ISSN: 1226-4601; 2055-7124

A novel approach for the sustained release film prepared by treating collagen solutions with different concentrations of carboxymethylcellulose (CMC) was evaluated as a biodegradable and biocompatible drug carrier for ophthalmic effects of the anti-proliferative drugs, 5-fluorouracil (5-FU) and mitomycinC (MMC). The influences of concentration of CMC (0, 0.025, 0.05, 0.1, 0.15, and 0.2% v/w) with a fixed concentration (10% v/w) of collagen on the cross-linking rate of collagen solutions and on the cross-linking extent of the collagen contained within were examined by monitoring in viscosity changes. In addition, the influences of the addition of drugs on viscosity change of collagen solutions were compared. The results demonstrate that the maximal viscosity of collagen solutions increases with increasing concentrations of CMC. When the concentration of CMC exceeds 0.1%, the maximal viscosity of collagen solutions reaches a plateau. However, drugs showed insignificant effects on viscosity changes of collagen solutions. The dissolution medium of the biodegraded films was assayed with HPLC method. Biodegradation studies and morphological characteristics of the films were also conducted in vitro using Coulter counter, SEM, and optical microscope. The collagen film exhibited sustained release effect and the release profile confirmed to the Higuchi equation. At most about 20% of the incorporated drugs were released over 21 days in a collagen-CMC film. Drug release was found to be effectively controlled by the drugs amount loaded in the collagen-CMC film. From these results, we postulated that it could be clinically used for prevention of posterior capsular opacification after cataract surgery. KCI Citation Count: 0