The Identification of a Receptor-binding Peptide Derived from Bone Morphogenetic Protein-6 and Its Role in Osteogenesis

• Published in: Biomaterials research pp. 8 - 17
• Main Authors: 최윤정, 이주연, Chong Pyoung Chung, 박윤정
• Format: Journal Article
• Language: English
• Published: 한국생체재료학회 01.03.2014
• Subjects: 의공학
• ISSN: 1226-4601; 2055-7124

Bone morphogenetic protein (BMP)-6 induces the differentiation of mesenchymal-derived cells into osteochondrogenic cells. Similar to the potent osteochondrogenic growth factor BMP-2, the BMP-6 also contains the “knuckle epitope”, which is thought to bind BMP receptor type II. In this study, we analyzed a synthetic peptide, YVPLPCCAPTKLNAISVLYF, namely BMP receptor binding peptide (BRBP), corresponding to residues of XX-YZ of the knuckle epitope of BMP-6. Our data revealed that the BRBP significantly binds to both type I and type II BMP receptors(BMPRs) with a similar affinity to BMP-6. The BRBP also induces osteogenic differentiation of human mesenchymal stem cells (HMSCs) with similar potency to BMP-6 as measured by alkaline phosphatase (ALP) activity, mineralization and osteogenic gene marker expression. Furthermore, the BRBP stimulates the phosphorylation of both the extracellular signal-regulated kinase (ERK1/2), and the Smad1/5/8 proteins. Blocking ERK-signaling with U0126, an inhibitor for MAPK/ERK kinase (MEK), abolishes the oseteogenic effects of the BRBP on human mesenchymal stem cells (HMSCs). Thus our study identifies an osteogenic peptide derived from BMP-6, which may be useful for ossified tissue regeneration. KCI Citation Count: 2