Lamination of microfibrous PLGA fabric by electrospinning a layer of collagenhydroxyapatite composite nanofibers for bone tissue engineering

• Published in: Biomaterials research pp. 99 - 110
• Main Authors: 권기완, Kailash Chandra Gupta, 정경혜, 강인규
• Format: Journal Article
• Language: English
• Published: 한국생체재료학회 01.09.2017
• Subjects: 의공학
• ISSN: 1226-4601; 2055-7124
• DOI: 10.1186/s40824-017-0097-3

Background: To mimic the muscle inspired cells adhesion through proteins secretion, the lamination of collagen–hydroxyapatite nanorod (nHA) composite nanofibers has been carried out successfully on polydopamine (PDA)-coated microfibrous polylactide-co-glycolide (PLGA) fabrics. The lamination of collagen-hydroxyapatite composite nanofibers on polydopamine-coated microfibrous PLGA fabrics was carried through electrospinning the solution of collagen containing L-glutamic acid-grafted hydroxyapatite nanorods (nHA-GA) at a flow rate of 1.5 mL/h and an applied voltage of 15 kV. Results: In comparison to pristine PLGA, dopamine-coated PLGA and collagen-hydroxyapatite composite nanofiber lamination has produced more wettable surfaces and surface wettability is found to higher with dopamine-coated PLGA fabrics then pristine PLGA. The SEM micrographs have clearly indicated that the lamination of polydopaminecoated PLGA fabric with collagen-hydroxyapatite composite nanofibers has shown increased adhesion of MC3T3E1 cells in comparison to pristine PLGA fabrics. Conclusion: The results of these studies have clearly demonstrated that collagen-nHA composites fibers may be used to create bioactive 3D scaffolds using PLGA as an architectural support agent. KCI Citation Count: 2