Effect of Cellular Zinc on the Regulation of C2-ceramide Induced Apoptosis in Mammary Epithelial and Macrophage Cell Lines

• Published in: Animal bioscience pp. 1741 - 1745
• Main Authors: S. E. Han, 이홍구, C. H. Yun, 홍중산, 김성학, 강상기, 김상훈, 조재순, S. H. Ha, 최윤재
• Format: Journal Article
• Language: English
• Published: 아세아·태평양축산학회 01.12.2005
• Subjects: 축산학
• ISSN: 2765-0189; 2765-0235

Zinc is a trace element that is associated with a stimulation of immune function and regulation of ion balance for livestock production. In this study, the effect of zinc as inhibitor to apoptosis-induced cells was examined in vitro using mammary epithelial cell line, HC11 and macrophage cell line, NCTC3749. Cell viability, measured by MTT assay, indicated that 10 g/ml of zinc had a negative impact on cellular activity and 50 ng/ml was chosen for further testing. Apoptosis was induced in cells treated with C2- ceramide in serum-free media. DNA fragmentation and gene expression of acidic sphingomyelinase (a gene responsible for the progress of apoptosis) were distinctively low in zinc treated cells compared with those in non-treated controls. In conclusion, zinc is involved in the regulation of cell proliferation and apoptosis in mammary epithelial cells and macrophages. KCI Citation Count: 2