B-cell-activating factor is a regulator of adipokines and a possible mediator between adipocytes and macrophages

• Published in: Experimental & molecular medicine pp. 1 - 8
• Main Authors: 김미영, 김도환, 도명술
• Format: Journal Article
• Language: English
• Published: 생화학분자생물학회 01.01.2013
• Subjects: 생화학
• ISSN: 1226-3613; 2092-6413

3T3-L1 adipocytes express the B-cell-activating factor (BAFF) and three different BAFF receptors (BAFF-Rs). Furthermore,BAFF expression is regulated by inflammatory modulators, such as tumor necrosis factor-a and rosiglitazone. Here we investigated the function of BAFF in 3T3-L1 adipocytes and RAW 264.7 macrophages. We examined adipokine expression in 3T3-L1 adipocytes treated with 10 ng ml1 BAFF. We also examined inflammatory molecule expression in RAW 264.7macrophages treated with 10 or 100 ng ml1 BAFF. We examined BAFF expression in the coculture of 3T3-L1 adipocytes and RAW 264.7 macrophages, as well as in white adipose tissue (WAT) of diet-induced obese (DIO) mice. We found that BAFF decreases leptin and adiponectin expression, but increases the expression of proinflammatory adipokines monocyte chemotactic protein-1, interleukin-6 (IL-6), cyclooxygenase-2 (COX-2) and haptoglobin. Coculturing the two cell types resulted in increased BAFF mRNA and protein expression, as well as modulation of BAFF-R mRNA expression in both cell types. These data indicate that BAFF might mediate adipocyte and macrophage interaction. When RAW 264.7 macrophages were treated with BAFF,BAFF-R expression was modulated as in coculture, and nitric oxide synthase and IL-6 expression increased. BAFF expression also increased in WAT of DIO mice. We propose that BAFF can regulate adipokine expression and possibly mediate adipocyte and macrophage interaction. KCI Citation Count: 11