1,3,5-Triazine계 제초제의 다중 제초작용성 분별을 위한 생물검정

• Published in: Weed & turfgrass science pp. 29 - 42
• Main Authors: 김진석, 김단희, Umurzokov Mirjalol, 박기웅, 임희남
• Format: Journal Article
• Language: Korean
• Published: 한국잔디학회 01.07.2025
• Subjects: 농학
• ISSN: 2287-7924; 2288-3312
• DOI: 10.5660/WTS.2025.14.2.1

본 연구에서는 s-triazine (1,3,5-triazine)계 화합물 180종을 대상으로 제초 작용생리의 유형과 구조활성 특징을 간단히 파악하여 보았다. 아울러 작용생리 유형을 보다 합리적으로 검정할 수 있는 새로운 검정체계의 구축 가능성을 검토하여 다중작용점을 가진 제초화합물들이 탐색될 수 있는지를 확인하였다. 그 결과, SPIPO 또는 LEMPA assay를 통해s-triazine계 화합물의 생물검정을 실시할 경우, Qy/VI 지수(SPIPO)나 Qy/GI 지수(LEMPA)를 활용하면 PS II 저해와 CBI/ Cell division저해제를단일검정으로손쉽게구별할수있으며,증상까지고려하면PS II, cell division, cellulose biosynthesis 저해제를 구별함과 동시에 다중 작용기작을 가진 화합물을 비교적 용이하게 선발해 낼 수 있을 것으로 여겨졌다. 한편얻어진작용기작에대한정보를바탕으로triazine골격의치환체와작용기작분화에대한상관관계를분석하였다.그결과 치환체 변화에 따른 liphophilicity 증감, 입체효과, 2번 탄소 아민 치환체 패턴이 작용기작의 분화에 크게 영향을 주는것을확인할수있었다.데이터가축적될시,작용기작을조절할수있는구조설계가가능하리라여겨진다. We investigated the types of herbicidal physiological actions and relationships between the structures and physiological actions of 180 1,3,5-triazine (s-triazine) derivatives. We also examined the possibility of establishing a new assay system that can more rationally verify the types of physiological actions required to detect herbicidal compounds with multiple mechanisms of action. Our findings showed that, when conducting bioassays of s-triazine compounds using Spirodela polyrhiza (SPIPO) or Lemna paucicostata (LEMPA) assays, the Quantum yield/Visual inhibition index (in SPIPO) or Quantum yield/Growth inhibition index (in LEMPA) allowed for easy differentiation of PS II inhibitors and cellulose biosynthesis/cell division inhibitors with a single test. Furthermore, by considering the symptoms, we were able to distinguish between PS II, cell division, and cellulose biosynthesis inhibitors and readily select compounds with multiple mechanisms of action. Based on the assay results regarding the mechanisms of action, we analyzed the correlation between the substituents of the triazine framework and the division of the mechanisms of action. The results demonstrate that the lipophilicity, steric effects, and substitution pattern of the C2-position amine significantly influenced the division of the mechanisms of action. It is expected that, with the accumulation of data, it may be possible to design structures by controlling the mechanisms of action. KCI Citation Count: 0