한우 수정란의 산소 소비량이 수정란이식 수태율에 미치는 영향

• Published in: Journal of animal reproduction & biotechnology (Online) pp. 145 - 148
• Main Authors: 최창용(농촌진흥청 축산기술연구소, 손준규(농촌진흥청 축산기술연구소, 조상래(농촌진흥청 축산기술연구소, 강다원, 연성흠, 최선호(농촌진흥청 축산기술연구소, 최수호, 김남태, 김재범, 정연섭, 정진우, 복난희, 유용희, 손동수
• Format: Journal Article
• Language: Korean
• Published: 사단법인 한국동물생명공학회 01.09.2010
• Subjects: 축산학
• ISSN: 2671-4639; 2671-4663

Oxygen consumption has been regarded as a useful indicator for assessment of mammalian embryo quality. This study was performed to investigate whether oxygen consumption reflects morphological grade of in vivo derived bovine blastocyst-stage embryos (blastocyst). The oxygen consumption of in vitro produced blastocyst was compared to its total cell number. In addition, pregnant rate was measured after transplantation of in vivo blastocysts with different oxygen consumption. The quality of blastocyst collected on day 7 after artificial insemination was categorized as grade Ⅰ and Ⅱ (G Ⅰ and G Ⅱ) based on microscopic observation of the morphology. Oxygen consumption of blastocyst was measured using a scanning electrochemical microscopy (SECM) and total cell number of in vitro blastocyst was enumerated by counting cells stained by propidium iodide. Pregnancy of recipient cow was confirmed with rectal palpation after 60 days of embryo transfer. The oxygen consumptions of G Ⅰ blastocysts were significantly higher than those of G Ⅱ blastocysts (10.2×1015/mol s-1 versus6.4×1015/mol s-1, p<0.05). Total cell numbers of in vitro blastocysts were 74.8, 90.7, and 110.2 in the oxygen consumption of below 10.0, 10.0～12.0, and over 12.0～1015/mol s-1, respectively. Total cell number was significantly increased in embryos with high oxygen consumption (p<0.05). Pregnant rate in recipient cow was 0, 50, and 85.7% in the transplantation of embryo with the oxygen consumption of below 10.0, 10.0～12.0, and over 12.0×1015/mol s, respectively. These results suggest that measurement of oxygen consumption may help increase the pregnant rate of bovine embryos. KCI Citation Count: 4