Automated measurements of tails in the single cell gel electrophoresis assay

This paper presents a method to automate a new technique, which has been developed to detect primary DNA damage of individual cells. It is based on single cell gel electrophoresis: the cells are placed in a gel, and then are prepared in order to free and unwind the DNA. After electrophoresis and sta...

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Bibliographic Details
Published in1996 IEEE Instrumentation and Measurement Technology Conference Proceedings Vol. 1; pp. 111 - 114 vol.1
Main Authors Rivest, J.-F., Tang, M., McLean, J., Johnson, F.
Format Conference Proceeding
LanguageEnglish
Published IEEE 1996
Subjects
DNA
Electric variables measurement
Electrokinetics
Image edge detection
Performance evaluation
Pixel
Protection
Robustness
Shape
Tail
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ISBN0780333128
9780780333123
DOI10.1109/IMTC.1996.507358

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Summary:This paper presents a method to automate a new technique, which has been developed to detect primary DNA damage of individual cells. It is based on single cell gel electrophoresis: the cells are placed in a gel, and then are prepared in order to free and unwind the DNA. After electrophoresis and staining, the resulting images appear as 'comets', the lighter fragments being at the tail end. Because small DNA fragments are pieces of damaged DNA strands, it is possible to assess DNA damage by measuring morphological characteristics of the comets.
ISBN:0780333128
9780780333123
DOI:10.1109/IMTC.1996.507358