Color fixed chromogenic analytical element

• Main Author: SHUENN-TZONG; CHEN
• Format: Patent
• Language: English
• Published: 30.11.1982
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TECHNICAL SUBJECTS COVERED BY FORMER USPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ARTCOLLECTIONS [XRACs] AND DIGESTS; TESTING; VINEGAR; WINE

Analytical element method of making the analytical element and process for determining an analyte in a body fluid sample are disclosed. More particularly, the method of preparing the element comprises the steps of: (a) impregnating a carrier with a first solution, having a pH not greater than about 2.5, which comprises a polymeric mordant and a tetraalkylbenzidine dihydrochloride, in a molar concentration greater than that of the polymeric mordant in an aqueous solvent and drying the carrier; (b) impregnating the carrier of (a) with a solution, having a pH of at least about 7.0, which comprises an analyte-responsive component in an aqueous solvent and drying the carrier. Optionally, there can be added the step of impregnating the carrier of (b) with a solution of a semi-permeable polymer in an organic solvent and drying the carrier.