DETECTION METHOD OF BCR-ABL INHIBITOR RESISTANCE-ASSOCIATED MUTATIONS, AND DATA ACQUISITION METHOD FOR PREDICTING BCR-ABL INHIBITOR RESISTANCE USING THE SAME

• Main Authors: ABE KENICHI, YUJIRI TOSHIAKI, OBA MITSUYOSHI, YAMANO HIROBUMI
• Format: Patent
• Language: English; Japanese
• Published: 16.05.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To detect mutations associated with BCR-ABL inhibitor resistance in chronic myeloid leukemia and the like with higher accuracy.SOLUTION: A step of amplifying a region comprising a fusion site comprised in BCR-ABL fusion genes and a mutation site involved in BCR-ABL inhibitor resistance using a biological specimen collected from a subject; and a step of determining the genotype of the mutation site involved in the above BCR-ABL inhibitor resistance using a nucleic acid amplified in the above step are comprised.SELECTED DRAWING: None 【課題】慢性骨髄性白血病等におけるBCR-ABL阻害剤耐性に関連する突然変異をより高精度に検出する。【解決手段】被験者から採取した生体試料を使用して、BCR-ABL融合遺伝子に含まれる融合部位及びBCR-ABL阻害剤耐性に関与する突然変異部位を含む領域を増幅する工程と、上記工程で増幅した核酸を用いて、上記BCR-ABL阻害剤耐性に関与する突然変異部位の遺伝子型を決定する工程とを含む。【選択図】なし