Biosensor

• Main Authors: VON GENTZKOW, WOLFGANG, WANNER, GERHARD, FEUCHT, HANS-DIETER, FORMANEK, HELMUT
• Format: Patent
• Language: English; French; German
• Published: 26.11.1997
• Edition: 6
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS BASED THEREON; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONSONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; ORGANIC MACROMOLECULAR COMPOUNDS; PEPTIDES; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; THEIR PREPARATION OR CHEMICAL WORKING-UP; VINEGAR; WINE

A biosensor having a selective detection system composed of a polymer and of a biochemical substance, in particular an enzyme, is characterised by a detection system produced in the following way: - an olefinically unsaturated, epoxy-functional polysiloxane is applied in the form of a film to a carrier material, - the polysiloxane is crosslinked by high-energy radiation to give a wide-mesh epoxy-functional polymer matrix, - the film is treated with an aqueous solution of the biochemical substance, resulting in immobilisation of the biochemical substance by reaction with epoxide groups in the polymer matrix, - the film is stabilised by reaction of unreacted epoxide groups with a compound containing amino and/or carboxyl groups.