一种肝脏响应的合成巨噬细胞及其构建方法和肿瘤免疫治疗应用

• Format: Patent
• Language: Chinese
• Published: 17.10.2025
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

本发明公开了一种肝脏响应的合成巨噬细胞及其构建方法和肿瘤免疫治疗应用，属于生物医药技术领域。该系统为稳定表达的αSLC17A2-P65/shSIRPα-synM系统的IBMDM细胞系，包括胞外段和胞内段，胞外段由识别抗原SLC17A2的单链抗体scFv和转录因子GAL4-VP16(GV)构成，胞内段由GV识别的UAS启动子序列和P65/shSIRPα构成。当抗体与肝脏发生响应时，通过synNotch的自切割作用释放GV，GV入核后识别UAS启动子序列，激活下游基因表达。本发明还提供了该系统的构建方法，包括构建载体质粒、慢病毒包装和目的细胞系构建三个步骤。该系统可应用于筛选抗肝癌免疫治疗药物，为肝癌治疗提供新的研究工具。