Method for establishing static cell three-dimensional model of colon cancer tumor based on matrix hardness

• Main Authors: JIA YIYANG, GUO YANTONG
• Format: Patent
• Language: Chinese; English
• Published: 05.11.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention is suitable for the technical field of bioengineering, and provides a method for establishing a three-dimensional model of colon cancer tumor static cells (CQCs) based on matrix hardness. Alginate is treated through high-pressure steam sterilization, a solution is prepared, after the solution is mixed with Matrigel and a cell suspension, CaCO3 turbid liquid is added to adjust the hardness, and finally the tumor spheres are formed through culture under specific conditions. The three-dimensional cell culture system which is uniform and transparent, controllable in gelation time, mild in environment and suitable for cell growth is constructed, and new possibility is provided for establishment of an in-vitro three-dimensional CQCs model; according to the invention, wide coverage of hardness from healthy colon tissue to colon cancer tissue is realized, the in-vivo environment can be simulated more truly, and the stability of the model is improved; the invention discovers and proves that the hard matr