一种慢性原发性疼痛小鼠模型的构建方法及应用

• Format: Patent
• Language: Chinese
• Published: 03.11.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW BREEDS OF ANIMALS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; TRAPPING

本发明公开了一种慢性原发性疼痛(CPP)小鼠模型的构建方法及应用，涉及实验动物模型技术领域。本发明慢性原发性疼痛小鼠模型的构建方法包括以下步骤：(1)通过测定小鼠机械痛阈基线水平筛选纳入模型构建的小鼠；(2)采用低频经皮电刺激(LF-PENS)作用于步骤(1)筛选得到的小鼠腘窝，经多种行为学测试验证后获得慢性原发性疼痛模型小鼠。本发明慢性原发性疼痛小鼠模型是采用简易且非侵入性电刺激操作构建。本发明慢性原发性疼痛小鼠模型的构建方法步骤简单，模型构建成功率高，对慢性原发性疼痛的病理研究及药物理疗等治疗方法手段的筛选和评估具有重要价值和意义。 The invention discloses a construction method and application of a chronic primary pain (CPP) mouse model, and relates to the technical field of experimental animal models. The construction method of the chronic primary pain mouse model comprises the following steps: (1) screening mice incorporated into model construction by measuring the mechanical pain threshold baseline level of the mice; (2) using low-frequency percutaneous electrical stimulation (LF-PENS) to act on the mouse popliteal fossa screened in the step (1), and obtaining the chronic primary pain model mouse after verification of various behavioral tests. The chronic primary pain mouse model disclosed by the invention is constructed by adopting simple and non-invasive electrical