Treatment of type 1 diabetes mellitus by M2 polarization of macrophages in pancreas

• Main Authors: GEERTS GERRIT, XIAO XIANGWEI
• Format: Patent
• Language: Chinese; English
• Published: 06.12.2022
• Subjects: HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS

A method for polarizing a macrophage into an M2 macrophage is disclosed. Methods for treating type 1 diabetes in a subject are also disclosed. The methods comprise administering to a subject a vector comprising a macrophage-specific promoter operably linked to a nucleic acid molecule encoding a TNF-alpha induced protein 8-like 2 (TIPE2) protein. In some embodiments, a carrier is topically administered to the pancreas of a subject. In a further embodiment, disclosed is a composition comprising a) a vector comprising a macrophage specific promoter operably linked to a nucleic acid molecule encoding a TNF-alpha induced protein 8-like 2 (TIPE2) protein; b) a buffer solution; and c) a contrast dye for endoscopic retrograde cholangiopancreatography. 公开了用于将巨噬细胞极化成M2巨噬细胞的方法。还公开了用于治疗受试者的1型糖尿病的方法。这些方法包括向受试者施用载体，该载体包含可操作地连接至编码TNF-α诱导的蛋白8样2(TIPE2)蛋白的核酸分子的巨噬细胞特异性启动子。在一些实施方案中，将载体局部施用至受试者的胰腺。在进一步的实施方案中，公开了组合物，该组合物包括a)载体，该载体包含可操作地连接至编码TNF-α诱导的蛋白8样2(TIPE2)蛋白的核酸分子的巨噬细胞特异性启动子；b)缓冲液；和c)用于内窥镜逆行胰胆管造影的造影染料。