Methods of amplifying T cells to treat cancer and related malignancies

• Main Authors: VILLARD YANNICK, WALTER STEFANIE, KALLA MARKUS, MATTA MEGAN, MOHAMMED ABDULLAH
• Format: Patent
• Language: Chinese; English
• Published: 02.12.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

An in vitro method of expanding [gamma] [delta] T cells comprises isolating [gamma] [delta] T cells from a blood sample of a human subject, activating the isolated [gamma] [delta] T cells in the presence of an amino bisphosphonate and/or feeder cells and at least one cytokine, expanding the activated [gamma] [delta] T cells, and optionally re-stimulating the expanded [gamma] [delta] T cells. 一种扩增γδT细胞的体外方法，包括从人体受试者的血液样本中分离γδT细胞、在存在氨基二膦酸盐和/或饲养细胞和至少一种细胞因子的情况下激活分离的γδT细胞、扩增激活的γδT细胞，以及任选地重新刺激扩增的γδT细胞。