NtNAC083基因在负调控烟草冷胁迫应答中的应用

• Format: Patent
• Language: Chinese
• Published: 03.11.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

本发明提供了一种NtNAC083基因在负调控烟草冷胁迫应答中的应用，属于基因调控技术领域。本发明发现NtNAC083基因可作为一个负调控因子参与植株对低温冷胁迫的响应，通过过表达株系地上部分冷表型发现，其有效降低了烟草植株对低温的耐受性；通过生理和分子实验数据进一步揭示NtNAC083基因可能通过调节冷胁迫关键基因CBF1或NCED3的表达来调节植物对低温的响应。该发现的获得对于研究烟草低温胁迫应答分子机理研究，通过基因工程方法培育耐低温抗早花新品种拓展了有效解决途径。 The invention provides application of an NtNAC083 gene in negative regulation of tobacco cold stress response, and belongs to the technical field of gene regulation. The invention finds that the NtNAC083 gene can be used as a negative regulatory factor to participate in the response of the plant to the low-temperature cold stress, and the cold phenotype of the overground part of the over-expressed strain finds that the tolerance of the tobacco plant to the low temperature is effectively reduced; physiological and molecular experimental data further reveals that the NtNAC083 gene can adjust the response of the plant to the low temperature by adjusting the expression of the cold stress key gene CBF1 or NCED3. The discovery expands an effective solution way for researching a low