一种含基质胶的硬度可调的水凝胶类器官球及其制备方法和应用

• Format: Patent
• Language: Chinese
• Published: 19.04.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

本发明公开了一种含基质胶的硬度可调的水凝胶类器官球及其制备方法和应用，属于类器官技术领域。所述的类器官球由基质胶、胶原、海藻酸盐等天然高分子材料形成的水凝胶构成，将细胞均匀分散到上述水凝胶溶液中形成水相，通过微流控油包水技术制备成粒径均一、单分散的液滴，再通过交联剂交联形成结构均一的载细胞互穿网络水凝胶类器官球。本发明的最大优势在于不改变基质胶和/或胶原含量的前提下，仅通过调节水相中海藻酸盐种类、浓度及交联剂种类，可以制备出硬度在100Pa-800kPa范围准确调控的水凝胶类器官球，用于模拟人体各种软组织微环境硬度，尤其可用于各种肿瘤类器官，为高通量药物筛选和临床个体化用药提供实验依据。 The invention discloses a hardness-adjustable hydrogel organoid sphere containing matrigel as well as a preparation method and application thereof, and belongs to the technical field of organoid. The organ-like sphere is composed of hydrogel formed by natural polymer materials such as matrigel, collagen and alginate, cells are uniformly dispersed into a hydrogel solution to form a water phase, and monodispersed liquid drops with uniform particle size are prepared by a microfluidic water-in-oil technology; and then cross-linking through a cross-linking agent to form the cell-loaded interpenetrating network hydrogel organ-like sphere with a uniform structure. Compared with the prior art, the preparation method has the