Method for simultaneously extracting nannochloropsis oculata protein and dietary fibers by ultrasonic assistance

• Main Authors: MA HAILE, SUN NIANZHEN, REN XIAOFENG, DING QINGZHI, SU XIAODONG, FEI SHILU, LU XINYI, RONG GEQING, XIONG TIANCHENG, LUO LIN, CHENG QIAOYU
• Format: Patent
• Language: Chinese; English
• Published: 10.09.2021
• Subjects: FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; PROTEIN COMPOSITIONS FOR FOODSTUFFS; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; WORKING-UP PROTEINS FOR FOODSTUFFS

The invention discloses a method for simultaneously extracting a nannochloropsis oculata protein and dietary fibers by ultrasonic assistance, and relates to the technical field of extraction of a protein and dietary fibers. The method comprises the steps of degreasing, wall breaking, double-enzyme enzymolysis, enzyme deactivation, solid-liquid separation, protein extraction, soluble dietary fiber separation, heavy metal removal and the like. The method disclosed by the invention is simple to operate, high in protein and dietary fiber extraction rate and low in heavy metal content, and three products including the nannochloropsis oculata protein, the nannochloropsis oculata soluble dietary fiber and the nannochloropsis oculata insoluble dietary fiber are simultaneously obtained in one technological process. 本发明利用超声波辅助同时提取微拟球藻蛋白和膳食纤维的方法，涉及蛋白和膳食纤维的提取技术领域。包括脱脂，破壁，双酶酶解，灭酶，固液分离，蛋白提取，分离可溶性膳食纤维，脱除重金属等步骤；本发明所述方法操作简单，蛋白、膳食纤维提取率高，重金属含量低，一个工艺过程同时得到微拟球藻蛋白、微拟球藻可溶性膳食纤维，微拟球藻不溶性膳食纤维三个产品。