测3种雌激素的化学衍生-超高效液相色谱-串联质谱法

本发明提供了一种同时检测血清中3种雌激素的化学衍生-超高效液相色谱-串联质谱法，采用一种新型衍生化试剂3-甲基-8-喹啉磺酰氯对目标雌激素分析物进行化学衍生后，用于超高效液相色谱-串联质谱检测。本发明所提供的方法采用3-甲基-8-喹啉磺酰氯对雌激素的酚羟基特异性衍生化，通过衍生化反应将质子化的带电荷基团引入目标雌激素分析物中，雌激素衍生物的pKa值增加，增强了离子化效率，提高了雌激素质谱检测的灵敏度；通过优化色谱条件，减少同分异构体共流出，降低基质干扰，提高了本方法定量的特异性、准确性和灵敏度；本方法具有操作简单、反应时间短、灵敏度高且无交叉污染、衍生产物稳定且衍生化试剂廉价易得的优势。 Th...

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Bibliographic Details
Format Patent
LanguageChinese
Published 06.12.2022
Subjects
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Summary:本发明提供了一种同时检测血清中3种雌激素的化学衍生-超高效液相色谱-串联质谱法，采用一种新型衍生化试剂3-甲基-8-喹啉磺酰氯对目标雌激素分析物进行化学衍生后，用于超高效液相色谱-串联质谱检测。本发明所提供的方法采用3-甲基-8-喹啉磺酰氯对雌激素的酚羟基特异性衍生化，通过衍生化反应将质子化的带电荷基团引入目标雌激素分析物中，雌激素衍生物的pKa值增加，增强了离子化效率，提高了雌激素质谱检测的灵敏度；通过优化色谱条件，减少同分异构体共流出，降低基质干扰，提高了本方法定量的特异性、准确性和灵敏度；本方法具有操作简单、反应时间短、灵敏度高且无交叉污染、衍生产物稳定且衍生化试剂廉价易得的优势。 The invention provides a chemical derivatization ultra-high performance liquid chromatography-tandem mass spectrometry method for simultaneously detecting three estrogens in serum, which is used for ultra-high performance liquid chromatography-tandem mass spectrometry detection after a novel derivatization reagent 3-methyl-8-quinoline sulfonyl chloride is adopted to carry out chemical derivatization on a target estrogen analyte. According to the method provided by the invention, 3-methyl-8-quinoline sulfonyl chloride is adopted for specific derivatization of phenolic hydroxyl groups of estrogen, protonated charged groups are introduced into a target estrogen analyte through a derivatization reaction, the pKa value of
Bibliography:Application Number: CN202011541839