AGENT AND METHOD FOR ENHANCING FERTILITY

• Main Authors: WALTON KELLY L, HARRISON CRAIG A
• Format: Patent
• Language: Chinese; English
• Published: 02.04.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

An agent capable of promoting proliferation and differentiation of granulosa cells is disclosed, which comprises a growth and differentiation factor-9 (GDF9) protein comprising a modified GDF9 polypeptide monomer which includes at least one amino acid substitution that enhances binding to and/or activation of activin-like kinase 4 and/or 5 receptor (ALK4/5). The agent is preferably provided in a mature dimeric form (eg comprising two monomers of the same modified GDF9 polypeptide monomer) and/or in a pro/mature complex form. The agent may be suitable for, inter alia, promoting oocyte maturation in vitro for use in assisted reproductive technologies. 公开了一种能够促进粒层细胞的增殖和分化的剂，所述剂包含生长和分化因子-9(GDF9)蛋白，所述蛋白包含包括至少一个使与活化素样激酶4和/或5受体(ALK4/5)的结合和/或活化素样激酶4和/或5受体(ALK4/5)的活化增强的氨基酸取代的经修饰GDF9多肽单体。所述剂优选以成熟二聚形式(例如包含同一经修饰GDF9多肽单体的两个单体)和/或以原/成熟复合物形式提供。所述剂可尤其适于在体外促进卵母细胞成熟以用于辅助生殖技术中。