护理点测定法

• Format: Patent
• Language: Chinese
• Published: 03.11.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

提供了一种适于护理点评估肝疾病或功能的免疫测定法，所述免疫测定法包括：使来自受试者的血液样品与识别ALT1的表位的特异性结合剂接触以形成抗原-结合剂复合物，并且使用与可检测报告物连接或包含可检测报告物的第二或进一步的结合剂检测所述复合物，当啮齿类抗体在人血浆的存在下时所述表位不被所述啮齿类抗体识别；以及依据所述样品中的ALT1的质量浓度，检测所述受试者中的肝疾病或肝功能。还提供了适于测量来自受试者的血液样品中的ALT1的质量浓度以测定肝功能的包含兔类动物抗体或含有其抗原结合组分的结合剂的试剂盒或装置。 An immunoassay suitable for point of care to assess liver disease or function comprising contacting a blood sample from a subject with a specific binding agent that recognizes an epitope of ALT 1, that is not recognized by rodent antibodies when the rodent antibodies are in the presence of human plasma, to form an antigen-binding agent complex and detecting the complex using a second or further binding agent linked to or comprising a detectable reporter; and detecting liver disease or liver function in the subject contingent upon the mass concentration of AL T1 in the sample. Kits or devices comprising lagomorph antibodies or binding agents comprising the antigen binding component thereof suitable for measuring the mass concentration of ALT 1 in a blood sample