使用固体对象的核酸提取方法

• Format: Patent
• Language: Chinese
• Published: 05.08.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRANSPORTING; VINEGAR; WINE

本发明涉及使用固体对象的核酸提取方法。相比传统核酸提取方法，通过在对象中形成核酸样品和亚氨酸酯化合物(imidoester compound)之间的复合物能够以更简单且成本更低的方式分离大量的和高纯度的核酸。特别是，与传统硅基材相比，用于提取核酸的薄膜设备具有改善的亲水性能，因此可更有效地提取核酸。 The present invention relates to a nucleic acids extraction method using a solid subject, and it can isolate nucleic acids with large amounts and high purity at a low cost and simpler than conventional methods of extracting nucleic acids by forming a complex of a nucleic acids sample and an imidoesters compound in the subject, and particularly, the thin film device used for extracting nucleic acids has improved hydrophilicity compared to the conventional silicon substrate, so that nucleic acids can be extracted more efficiently.