利用L-脯氨酸诱导培养提高酵母抑制果实病害效力的方法、制剂及所用培养基

• Format: Patent
• Language: Chinese
• Published: 19.08.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FOODS OR FOODSTUFFS; HUMAN NECESSITIES; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT,VEGETABLES, EDIBLE SEEDS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THE PRESERVED, RIPENED, OR CANNED PRODUCTS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE

本发明提供一种提高生防酵母对果实病害防治效力的培养基，包括活化培养基和种子培养基；活化培养基为：牛肉浸膏8g、酵母粉5g、葡萄糖10g、琼脂20g、L-脯氨酸0～1000mg(优选100mg)，加水定容至1000mL，灭菌后得NYDA活化培养基；种子培养基为：牛肉浸膏8g、酵母粉5g、葡萄糖10g、L-脯氨酸0～1000mg(优选100mg)，加水定容至1000mL，灭菌后得NYDB种子培养基。本发明还提供一种诱导提高生防酵母对果实病害防治效力的制剂，包括活化、液体培养和离心分离；本发明使用L-脯氨酸培养罗伦隐球酵母控制梨果实青霉病的发病率，使用方法简单，操作方便，效果好，成本低。 The invention provides a culture medium for improving the effect of inhibiting fruit diseases by yeast, which comprises an activation culture medium and a seed culture medium; the activation culture medium comprises 8g of beef extract, 5g of yeast powder, 10g of glucose, 20g of agar, and 0-1000mg of L-proline (preferably 100mg), water is added to a constant volume of 1000mL, and sterilizing is carried out to obtain the NYDA activation culture medium; the seed culture medium comprises 8g of beef extract, 5g of yeast powder, 10g of glucose, 0-1000mg of L-proline (preferably 100mg), water is added to a constant volume of 1000mL, and sterilizing is carried out to obtain the NYDB seed culture medium. The invention also provi