用于HCV核心抗原的快速检测的预处理方法

• Format: Patent
• Language: Chinese
• Published: 26.08.2022
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

本发明涉及检测来自受试者的样品中丙型肝炎病毒（HCV）的核心多肽的方法，所述方法包括（a）使所述样品与包含阳离子去污剂的表面活性剂接触；（b）使所述样品与结合化合物接触；和（c）检测所述样品中所述HCV的核心多肽；其中步骤a）后面紧接着是步骤b）。本发明进一步涉及预处理来自受试者的样品以用于检测HCV核心多肽的方法，包括（a）使所述样品与包含阳离子去污剂的表面活性剂并任选与诱导pH移动的试剂接触，后面紧接着是（b）使所述样品与结合化合物接触。此外，本发明进一步涉及与前述方法有关的用途、设备和分析系统。 The present disclosure relates to a method for detecting a core polypeptide of a hepatitis C virus (HCV) in a sample from a subject with the steps of (a) contacting the sample with a surfactant comprising a cationic detergent; (b) contacting the sample with a binding compound; and (c) detecting a core polypeptide of the HCV in the sample; wherein step a) is immediately followed by step b). The present disclosure further relates to a method for pre-processing a sample from a subject for detection of an HCV core polypeptide, involving (a) contacting the sample with a surfactant comprising a cationic detergent and, optionally, with an agent inducing a pH shift, immediately followed by (b) contacting the sample with a binding compound. Moreover, the present disclo