种用于表达罗伦隐球酵母Rho1GTP蛋白的酿酒酵母基因工程菌、其构建方法及其应用

• Format: Patent
• Language: Chinese
• Published: 05.02.2019
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

本发明涉及基因工程领域，特别涉及种用于表达罗伦隐球酵母Rho1 GTP蛋白的酿酒酵母基因工程菌、其构建方法及其应用。种用于表达罗伦隐球酵母rho1 GTP蛋白的酿酒酵母基因工程菌，其含有如SEQ ID NO：3所述的rho1基因cDNA序列。本发明将罗伦隐球酵母(Cryptococcus laurentii)rho1基因cDNA序列连接到pYES6/CT质粒载体上，并将重组载体转化到酿酒酵母(Saccharomyces cerevisiae)中进行高效的表达，实现了rho1基因过表达对于酿酒酵母抗逆性和生防效力的提高作用。 The invention relates to the field of genetic engineering, in particular to a saccharomyces cerevisiae genetically-engineered bacterium for expressing cryptococcus laurentii Rho1GTP protein, as well as a building method and an application of the saccharomyces cerevisiae genetically-engineered bacterium. The saccharomyces cerevisiae genetically-engineered bacterium for expressing the cryptococcus laurentii Rho1GTP protein contains a rho1 gene cDNA sequence presented as SEQ ID NO: 3. According to the saccharomyces cerevisiae genetically-engineered bacterium, the cDNA sequence of the cryptococcus laurentii rho1 gene is connected to a pYES6/CT plasmid vector, and the recombinant vector is transformed to the saccharomyces cerevisiae for efficient express