舞毒蛾热激蛋白Hsp23基因及其dsRNA在无公害防治中的应用

• Format: Patent
• Language: Chinese
• Published: 15.09.2017
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

本发明公开了种舞毒蛾热激蛋白Hsp23基因及其dsRNA在无公害防治中的应用。所述Hsp23基因的核苷酸序列和编码氨基酸序列如SEQ ID No.1所示，由该基因部分序列片段合成dsRNA，其序列如SEQ ID No.2所示。Hsp23基因dsRNA可应用在抑制舞毒蛾生长发育中。实验结果表明：当舞毒蛾幼虫注射Hsp23基因dsRNA后，与对照(注射ddHO和GFP基因dsRNA)相比均有较强的沉默效果，致使舞毒蛾幼虫生长发育受到抑制。因此本发明提出可利用Hsp23的沉默技术来防治林木重要害虫舞毒蛾及具有相似结构域的鳞翅目害虫，为绿色防治林业害虫提供了个新的思路和技术。