叶黄素-DHA复合微胶囊制备工艺的优化 及其储存稳定性和释放性能的研究 Optimization of preparation process of lutein-DHA composite microcapsules and their storage stability and release properties

• Published in: Zhongguo you zhi Vol. 50; no. 6; pp. 115 - 122,133
• Main Author: 顾庆琪，吴宇芳，陈天阳，王梦晶，王崴，李书红 GU Qingqi, WU Yufang, CHEN Tianyang, WANG Mengjing, WANG Wei, LI Shuhong
• Format: Journal Article
• Language: English
• Published: 中粮工科（西安）国际工程有限公司 01.06.2025
• Subjects: microcapsules; lutein; dha; complex coacervation; 微胶囊；叶黄素；dha；复凝聚法
• ISSN: 1003-7969
• DOI: 10.19902/j.cnki.zgyz.1003-7969.240586

为提高叶黄素和DHA在人体中的生物利用率，以叶黄素与DHA藻油为芯材，壳聚糖与羧甲基纤维素为壁材，采用复凝聚法制备叶黄素-DHA复合微胶囊，采用单因素实验与正交实验对叶黄素-DHA复合微胶囊制备工艺进行优化，并考察其储存稳定性和体外释放性能。结果表明：叶黄素-DHA复合微胶囊最佳制备工艺为pH 3.8、壁材质量浓度1.0 g/100 mL（壳聚糖与羧甲基纤维素质量比1∶ 1）、单双甘油酯用量0.7%（以DHA藻油质量计）、芯壁比1∶ 2，在此条件下DHA包埋率为84.83%，叶黄素包埋率为82.71%，微胶囊产率为87.26%；与游离叶黄素相比，叶黄素-DHA复合微胶囊展现出高的热稳定性和低的光解速率；在体外模拟释放实验中，叶黄素-DHA复合微胶囊在模拟胃液中经2 h消化后，叶黄素的相对累计释放率为23.70%，DHA的相对累计释放率为2045%，而在模拟肠液中经4 h消化后，叶黄素的相对累计释放率为77.78%，DHA的相对累计释放率为84.09%。综上，叶黄素-DHA复合微胶囊可提高叶黄素和DHA的储存稳定性和缓释性能。 In order to improve the bioavailability of lutein and DHA in human body, lutein-DHA composite microcapsules were prepared by complex coacervation method with lutein and DHA as the core material, and chitosan and carboxymethylcellulose as the wall material, and the process of lutein-DHA composite microcapsules was optimized by single factor and orthogonal experiments and the storage stability and in vitro release properties were investigated. The results showed that the optimal preparation process for lutein-DHA composite microcapsules was pH 3.8, wall material mass concentration 1.0 g/100 mL (mass ratio of chitosan to carboxymethylcellulose 1∶ 1), mono-diglycerides dosage 0.7%(according to mass of DHA algal oil), and core-to-wall ratio 1∶ 2. Under these conditions, the embedding rate of DHA was 84.83%, the embedding rate of lutein was 82.71%, and the microcapsule yield was 8726%. Lutein-DHA composite microcapsules exhibited high thermal stability and low photolysis rates compared to free lutein. In the in vitro simulated release experiments, the relative cumulative release rate of lutein-DHA composite microcapsules was 23.70% for lutein and 20.45% for DHA after 2 h of digestion in simulated gastric fluid, whereas the relative cumulative release rate of lutein was 77.78% for lutein and 84.09% for DHA after 4 h of digestion in simulated intestinal fluid. In conclusion, lutein-DHA composite microcapsules can improve the storage stability and slow release properties of lutein and DHA.