基于DB-FastFAME色谱柱的气相色谱法 测定食用植油脂肪酸组成Determination of fatty acid composition of edible vegetable oil by gas chromatography based on DB-FastFAME column

• Published in: Zhongguo you zhi Vol. 50; no. 5; pp. 150 - 152
• Main Author: 孟庆宇，蒋甜燕,孙日飞，马莉，夏旭明 MENG Qingyu, JIANG Tianyan, SUN Rifei, MA Li, XIA Xuming
• Format: Journal Article
• Language: English
• Published: 中粮工科（西安）国际工程有限公司 01.05.2025
• Subjects: fatty acid composition; db-fastfame column; resolution; precision; accuracy; 脂肪酸组成；db-fastfame色谱柱；分离度；精密度；准确度
• ISSN: 1003-7969
• DOI: 10.19902/j.cnki.zgyz.1003-7969.240029

为满足食用植物油生产企业脂肪酸组成测定及时性、准确性的要求，采用气相色谱法，选用强极性DB-FastFAME色谱柱对食用植物油的脂肪酸组成进行测定，并对方法的分离度、精密度、准确度及柱效能进行分析。结果表明：采用DB-FastFAME色谱柱可在25 min内分离37种脂肪酸甲酯混标，分离度均不小于1.5，脂肪酸的定性和定量相对标准偏差（RSD）分别小于0.03%和2.3%，分离效果良好且稳定性较好；采用该色谱柱测定菜籽油质控样品的脂肪酸组成，相对误差为-2.50%~9.01%，无法分离反式C18∶ 3，除C18∶ 3n-3和C20∶ 1测定值偏大外，其余脂肪酸准确度良好；DB-FastFAME色谱柱使用1年后，柱效能降低，保留时间后移了0.9 min。综上，DB-FastFAME色谱柱可满足企业低成本、高效率的脂肪酸组成分析要求，但长期使用需关注柱效能变化。 In order to meet the requirements of timeliness and accuracy in the detection of fatty acid composition in edible vegetable oil production enterprises, the fatty acid composition of edible vegetable oil was determined by gas chromatography method with highly polar DB-FastFAME column. The method resolution, precision, accuracy and column efficiency were analyzed. The results showed that the DB-FastFAME column could separate 37 fatty acid methyl esters mixed standard within 25 min, and the resolutions were all no less than 1.5, the relative standard deviation (RSD) was less than 0.03% for qualitation and less than 2.3% for quantitation, which indicated that the column had good separation effect and good stability. The fatty acid composition of rapeseed oil quality control sample was determined by this method, and the relative error ranged from -2.50% to 9.01%, which was unable to separate trans C18∶ 3, and the accuracy of the fatty acids was good, except for the measured value of C18∶ 3n-3 and C20∶ 1 being high. After using for 1 year, the column efficiency of DB-FastFAME column decreased and the retention time shifted by 0.9 min. In conclusion, DB-FastFAME column can meet the enterprises requirements of low-cost and high-efficiency fatty acid composition analysis, but the long-term use should be concerned about the column efficiency.