Characterization of the novel Sezary lymphoma cell line BKP 1

• Published in: Experimental dermatology Vol. 24; no. 1; pp. 60 - 62
• Main Authors: Boudjarane, John, Essaydi, Arnaud, Farnault, Laure, Popovici, Cornel, Lafage‐Pochitaloff, Marina, Beaufils, Nathalie, Berda‐Haddad, Yaël, Lacroix, Romaric, Nicolino‐Brunet, Corinne, Le Treut, Thérèse, Zattara, Hélène, Gabert, Jean, Kahn‐Perlès, Brigitte, Costello, Régis
• Format: Journal Article
• Language: English
• Published: 01.01.2015
• ISSN: 0906-6705; 1600-0625
• DOI: 10.1111/exd.12567

Cutaneous T‐cell lymphomas (CTCL) are a heterogeneous group of lymphomas primarily involving the skin. The most common types are mycosis fungoides (MF) and Sezary Syndrome (SS). We report a novel long‐term fast‐growing SS line termed BKP1 that was characterized by flow cytometry (FC), conventional and molecular cytogenetic [FISH/multi‐FISH together with array comparative genomic hybridization ( aCGH )]. FC immunophenotype of the BKP1 is CD2+CD5+CD3+CD4+CD8−CD7−CD25−CD26−CD30−CD158k+. The TCR γ characterization of BKP1 by PCR identified a clonal rearrangement. The conventional cytogenetic and Multi‐FISH analysis showed complex chromosomal rearrangements. aCGH analysis highlighted the loss of genes involved in cell cycle control, in immune response ( HLA , complement complex) and DNA damage repair mechanisms. The BKP1 is another lymphoma cell line thoroughly characterized that can be a valuable tool for both basic and applied research such as identification of deregulated genes and/or pathways and screening for new antilymphoma drugs.