基于Ru（bpy）32＋/AuNPs/SWCNTs/腺苷适配体电化学发光生物传感器用于腺苷的检测

利用AuNPs/Nafion复合膜技术固定Ru（bpy）32＋,采用羧基化碳纳米管固定氨基化腺苷适配体,制备腺甘电化学发光生物传感器。采用循环伏安法和电化学发光法对传感器进行表征。结果表明,此传感器具有良好的稳定性和重现性。腺苷与传感器作用后,腺苷与其适配体形成G四面体结构,Ru（bpy）32＋的电化学发光强度降低。在最佳实验条件下,电化学发光强度降低量与腺苷浓度的负对数在1.0×10 -111.0×10-7mol/L范围内呈良好的线性关系,线性方程为ΔIECL=-890lg C-5050,检出限（S/N=3）为5.0×10-（12）mol/L。对1.0×10-10mol/L腺苷平行测定11次...

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Published in分析化学 Vol. 45; no. 9; pp. 1353 - 1359
Main Author 樊雪梅 王书民 李哲建 贾雪艳 郑行望
Format Journal Article
LanguageChinese
Published 2017
Subjects
电化学发光
腺苷
腺苷适配体
Online AccessGet full text
ISSN0253-3820

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Summary:利用AuNPs/Nafion复合膜技术固定Ru（bpy）32＋,采用羧基化碳纳米管固定氨基化腺苷适配体,制备腺甘电化学发光生物传感器。采用循环伏安法和电化学发光法对传感器进行表征。结果表明,此传感器具有良好的稳定性和重现性。腺苷与传感器作用后,腺苷与其适配体形成G四面体结构,Ru（bpy）32＋的电化学发光强度降低。在最佳实验条件下,电化学发光强度降低量与腺苷浓度的负对数在1.0×10 -111.0×10-7mol/L范围内呈良好的线性关系,线性方程为ΔIECL=-890lg C-5050,检出限（S/N=3）为5.0×10-（12）mol/L。对1.0×10-10mol/L腺苷平行测定11次,相对标准偏差为2.7%。用于尿液中腺苷的测定,加标回收率在97.1%110.0%之间。
Bibliography:22-1125/O6
Electrogenerated chemiluminescence;Adenosine aptamer;Adenosine
Based on the Au NPs/Nafion composite membrane technology and immobilization of amino adenosine aptamer using carboxyl carbon nanotubes on the surface of a glassy carbon electrode, a electrochemiluminescence sensor was preparated. The sensor was characterized by cyclic voltammetry and electrochemical luminescence. The result showed that the sensor had a good stability and reproducibility.Adenosine and adenosine aptamer could form G-tetrahedral structure,leading a decrease of ECL intensity.Under the optimum experimental conditions,the relative ECL intensity showed a good linear relationship to the negative logarithm of adenosine concentration in the range of 1. 0 ×10-11-1. 0 ×10-7mol/L,the linear equations was ΔIECL= -890lg C-5050 with a detection limit of 5. 0 × 10-12mol/L. The RSD was 2. 7% in 11 times measurement of adenosine（ 1. 0 ×10-10mol/L）. The recovery was 97. 1%-110. 0% in the determination of real adenosine sample.
FAN Xue-Mei1, W
ISSN:0253-3820