基于核酸适体传感器的黄曲霉毒素B1检测研究进展

食物中存在毒性和致癌性较强的黄曲霉毒素B1（AFB1）,目前常规的检测方法有薄层色谱法、高效液相色谱法、液相色谱-串联质谱法和免疫分析法,但都存在一定局限性.基于核酸适体传感器的AFB1检测研究取得了一定成果,基于电化学核酸适体传感器、光学核酸适体传感器和PCR核酸适体传感器的3种新型检测技术各有利弊,将血糖仪作为检测仪器、与核酸适体传感器相结合检测AFB1的思路有待实验验证.未来基于生物识别元件和磁性材料的磁亲和固相萃取法与核酸适体传感器相结合进行AFB1检测,有望获得突破....

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Bibliographic Details
Published in轻工学报 Vol. 32; no. 2; pp. 40 - 47
Main Author 姜利英 刘帅 张培 任林娇 闫艳霞 郑晓婉 陈青华 姜素霞
Format Journal Article
LanguageChinese
Published 2017
Subjects
样品预处理
核酸适体传感器
血糖仪
黄曲霉毒素B1
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ISSN2096-1553

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Summary:食物中存在毒性和致癌性较强的黄曲霉毒素B1（AFB1）,目前常规的检测方法有薄层色谱法、高效液相色谱法、液相色谱-串联质谱法和免疫分析法,但都存在一定局限性.基于核酸适体传感器的AFB1检测研究取得了一定成果,基于电化学核酸适体传感器、光学核酸适体传感器和PCR核酸适体传感器的3种新型检测技术各有利弊,将血糖仪作为检测仪器、与核酸适体传感器相结合检测AFB1的思路有待实验验证.未来基于生物识别元件和磁性材料的磁亲和固相萃取法与核酸适体传感器相结合进行AFB1检测,有望获得突破.
Bibliography:JIANG Li-ying,LIU Shuai,ZHANG Pei,REN Lin-jiao,YAN Yan-xia, ZHENG Xiao-wan,CHEN Qing-hua,JIANG Su-xia（College of Electric Information Engineering, Zhengzhou University of Light Industry,Zhengzhou 450002, China）
aflatoxin B1; nucleic acid aptasensor; glucose meter; sample pretreatment
There is a high toxicity and carcinogenicity of aflatoxin B1（AFB1） in food.Current detection of aflatoxin B1 is performed by a variety of methods including thin layer chromatography,high performance liquid chromatography,liquid chromatography-tandem mass spectrometry and immunoassay,but they all have some limitations.AFB1 detection research had obtained certain achievements based on nucleic acid aptasensor.The three new detection technology based on the electrochemical nucleic acid aptasensor,optical nucleic acid aptasensor and PCR nucleic acid aptasensor had advantages and disadvantages.A new idea in which glucose meter was used as a detection instrument and combined with nucleic acid aptasensor for AFB1 qualitative analysis were
ISSN:2096-1553