炎症诱导早产小鼠脑组织长链非编码RNA的筛选及验证研究

目的探讨长链非编码RNA（lnc RNA）与炎症诱导早产小鼠脑损伤的关系,为脑损伤的防治提供依据。方法孕鼠腹腔注射LPS建立炎症诱导早产小鼠脑损伤模型（早产组）,正常孕鼠分娩足月小鼠作为对照（足月组）。应用lnc RNA芯片技术筛选与早产小鼠脑损伤相关的lnc RNA,并应用Real-time PCR技术对lnc RNA进行验证。结果比较早产组与足月组,显著差异表达的lnc RNA有1 978条（P〈0.05）,包括上调lnc RNA 786条,下调lnc RNA 1 192条,其中差异表达1.5倍及以上的lnc RNA共有29条。对差异表达倍数最高的前10条lnc RNA行进一步分析,发现这...

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Published in中国当代儿科杂志 Vol. 18; no. 5; pp. 435 - 439
Main Author 陈茹娟 奚莎 王凡 肖谧 林晓洁 刘俐
Format Journal Article
LanguageChinese
Published 2016
Subjects
G蛋白偶联受体
小鼠
炎症
长链非编码RNA
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ISSN1008-8830

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Summary:目的探讨长链非编码RNA（lnc RNA）与炎症诱导早产小鼠脑损伤的关系,为脑损伤的防治提供依据。方法孕鼠腹腔注射LPS建立炎症诱导早产小鼠脑损伤模型（早产组）,正常孕鼠分娩足月小鼠作为对照（足月组）。应用lnc RNA芯片技术筛选与早产小鼠脑损伤相关的lnc RNA,并应用Real-time PCR技术对lnc RNA进行验证。结果比较早产组与足月组,显著差异表达的lnc RNA有1 978条（P〈0.05）,包括上调lnc RNA 786条,下调lnc RNA 1 192条,其中差异表达1.5倍及以上的lnc RNA共有29条。对差异表达倍数最高的前10条lnc RNA行进一步分析,发现这些lnc RNA可能参与转录、信号转导、凋亡、细胞周期、炎症反应等生物学过程,并与G蛋白偶联受体信号通路、神经肽信号通路有关。对其中2条lnc RNA在早产组和足月组脑组织中的表达进行Real-time PCR验证,发现与芯片结果一致。结论炎症诱导早产小鼠脑组织中的lnc RNA表达谱发生了明显变化,G蛋白偶联受体信号通路可能参与早产脑损伤的发生机制。
Bibliography:Long non-coding RNA; Inflammation; G protein-coupled receptor; Mice
Objective To investigate the association between long non-coding RNAs（lnc RNAs）and brain injury in inflammation-induced preterm mice,and to provide a reference for the prevention and treatment of brain injury.Methods An intraperitoneal injection of lipopolysaccharide in pregnant mice was performed to establish a model of inflammation-induced preterm mice with brain injury（preterm group）.The full-term mice delivered by normal pregnant mice were used as controls（full-term group）.The lnc RNA chip assay was used to screen out the lnc RNAs associated with brain injury in preterm mice.Quantitative real-time PCR was used to validate the lnc RNAs identified by the above method.Results The preterm and full-term groups showed significant differences in the expression of 1 978 lnc RNAs（P〈0.05）,consisting of 786 up-regulated lnc RNAs and 1 192 down-regulated lnc RNAs,and 29 lnc RNAs were 1.5 or more times differentially expressed between the two groups.A
ISSN:1008-8830