rhPTH（1-34）诱导角质形成细胞蛋白酪氨酸激酶差异表达蛋白的筛选

目的利用蛋白芯片技术,筛选重组人甲状旁腺素（1-34）[rh PTH（1-34）]作用角质形成细胞后,156种蛋白酪氨酸激酶中的差异表达蛋白。方法培养Ha Ca T角质形成细胞株,rh PTH（1-34）诱导细胞,经过总蛋白质提取、BCA蛋白质定量、酪氨酸磷酸化抗体检测、图象和数据采集,筛选出差异蛋白,最后对所得数据进行分析。结果 156种蛋白中,rh PTH（1-34）组与空白对照组比较,20种蛋白表达差异2倍以上,其中16种显著上调,分别为：Csk,Dtk,Eph A4,Eph A5,Eph B2,Eph B3,Erb B4,FAK,Insulin R,LTK,PDGFR-α,ROS,Ti...

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Published in中国皮肤性病学杂志 Vol. 30; no. 3; pp. 221 - 224
Main Author 卜晓琳 毕新岭 顾军 汪五清 史玉玲 徐晓光 王国江
Format Journal Article
LanguageChinese
Published 2016
Subjects
差异表达蛋白
蛋白酪氨酸激酶
角质形成细胞
重组人甲状旁腺素（1-34）
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ISSN1001-7089

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Summary:目的利用蛋白芯片技术,筛选重组人甲状旁腺素（1-34）[rh PTH（1-34）]作用角质形成细胞后,156种蛋白酪氨酸激酶中的差异表达蛋白。方法培养Ha Ca T角质形成细胞株,rh PTH（1-34）诱导细胞,经过总蛋白质提取、BCA蛋白质定量、酪氨酸磷酸化抗体检测、图象和数据采集,筛选出差异蛋白,最后对所得数据进行分析。结果 156种蛋白中,rh PTH（1-34）组与空白对照组比较,20种蛋白表达差异2倍以上,其中16种显著上调,分别为：Csk,Dtk,Eph A4,Eph A5,Eph B2,Eph B3,Erb B4,FAK,Insulin R,LTK,PDGFR-α,ROS,Tie-1,Tie-2,Tyk2,TYRO10;4种显著下调,分别为：EGFR,JAK3,ALK,Eph B1。其中与银屑病相关的EGFR,JAK3,Tyk2,Eph A4主要涉及角质形成细胞增殖、分化,调节T细胞介导的炎症反应。结论 rh PTH（1-34）可影响Ha Ca T细胞内一组蛋白酪氨酸激酶的表达,其中EGFR,JAK3,Tyk2,Eph A4与银屑病的发病机制密切相关,值得进一步深入探讨。
Bibliography:Keratinocytes; Parathyroid hormone; Protein tyrosine kinase; Differentially expressed proteins
BU Xiao-lin,BI Xin-ling,GU Jun,WANG Wu-qing,SHI Yu-ling,XU Xiao-guang,WANG GUO-jiang（ 1. Department of Dermatology, Shanghai Gongli Hospital, Secondary Military Medical University, Shanghai 200135, China ; 2. Department of Dermatology, Changhai Hospital, Secend Military Medical University, Shanghai 200433, China; 3. Depart- ment of Dermatology, Tenth Peaple＇s Hospital of Tongji University, Shanghai 200072, China ;4. Department of Dernuttology, Central Hospital of Shanghai Minhang District, Shanghai 201100, China;5. Department of Dermatology ,Zhoupu Hospital, Shanghai 201318, China）
Objective To screen the differentially expressed proteins of 156 kinds of protein tyrosine kinase in rh PTH（1-34）-induced keratinocytes using protein array. Methods We cultured keratinocyte cell line Ha Ca T. Cells were divided into two groups,rh PTH（ 1-34） treatment group and blank control group. Total proteins were extracted. BCA protein
ISSN:1001-7089