SRBSDV侵染的白背飞虱中肠酵母双杂交cDNA文库的构建和分析

南方水稻黑条矮缩病毒（Southern rice black-streaked dwarf virus,SRBSDV）是呼肠孤病毒科（Reoviridae）斐济病毒属（Fijivirus）成员,主要由白背飞虱（Sogatella furcifera,WBPH）以持久增殖型方式进行传播。在病毒的侵染循回过程中,白背飞虱中肠上皮细胞是病毒的初侵染和主要增殖场所。而病毒的有效增殖是决定介体能否传毒的关键影响因素。为了更好地研究SRBSDV和介体白背飞虱的互作关系,尤其是了解白背飞虱中肠蛋白通过参与调控病毒的增殖过程,而使介体昆虫成功获毒并传毒,本研究以高带毒白背飞虱群体中肠组织为实验材料,构建了高带...

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Bibliographic Details
Published in农业生物技术学报 Vol. 24; no. 2; pp. 245 - 251
Main Author 郑立敏 彭静 陈建斌 张松柏 程菊娥 张德咏 刘勇
Format Journal Article
LanguageChinese
Published 2016
Subjects
中肠
南方水稻黑条矮缩病毒（SRBSDV）
白背飞虱
酵母双杂cDNA文库
Online AccessGet full text
ISSN1674-7968

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Summary:南方水稻黑条矮缩病毒（Southern rice black-streaked dwarf virus,SRBSDV）是呼肠孤病毒科（Reoviridae）斐济病毒属（Fijivirus）成员,主要由白背飞虱（Sogatella furcifera,WBPH）以持久增殖型方式进行传播。在病毒的侵染循回过程中,白背飞虱中肠上皮细胞是病毒的初侵染和主要增殖场所。而病毒的有效增殖是决定介体能否传毒的关键影响因素。为了更好地研究SRBSDV和介体白背飞虱的互作关系,尤其是了解白背飞虱中肠蛋白通过参与调控病毒的增殖过程,而使介体昆虫成功获毒并传毒,本研究以高带毒白背飞虱群体中肠组织为实验材料,构建了高带毒白背飞虱群体中肠的酵母双杂交c DNA文库。经过检测表明,构建的文库滴度为1.5×106cfu/m L,平均插入片段主要分布在1.0~2.0 kb之间,文库质量较好,可用于研究SRBSDV编码蛋白和白背飞虱中肠蛋白的互作关系,并为开展SRBSDV和昆虫介体的互作研究奠定了基础。
Bibliography:ZHENG Li-Min,PENG Jing,CHEN Jian-Bin,ZHANG Song-Bai,CHENG Ju-E,ZHANG De-Yong,LIU Yong（Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, China）
11-3342/S
Southern rice black-streaked dwarf virus（SRBSDV）; White backed planthopper; Midgut; Yeast two-hybrid cDNA library
Southern rice black-streaked dwarf virus（SRBSDV）,the genus Fijivirus in the family Reoviridae,is transmitted mainly by white backed planthopper（Sogatella furcifera,WBPH） in a persistent- propagative manner. The epithelial cells of the midgut is the initially infected and replicated tissue for SRBSDV during the whole infection route in its insect vector. The effective multiplication of the virus is the key factor to determine whether the virus can be transmitted. In this study,to investigate the interaction between SRBSDV and its insect vector,and the mechanism of midgut protein regulating viral proliferation to transmit virus successfully. The yeast two-hybrid c DNA library for the midgut of SRBSDV-infected WBPH
ISSN:1674-7968