番茄中受TYLCV诱导上调表达基因tetraspanin3的功能分析

[目的]本文旨在研究tetraspanin3基因在抗番茄黄化曲叶病毒（TYLCV）病中的作用及对番茄生长发育的影响.[方法]通过MEG 5.0软件构建进化树,分析tetraspanin3基因的进化;采用实时荧光定量PCR（RT-qPCR）检测tetraspanin3在番茄各个器官中的表达量及对TYLCV诱导的响应;通过病毒诱导基因沉默技术（VIGS）将番茄中的tetraspanin3沉默,测定基因沉默后番茄的抗病性并观测沉默植株表型.[结果]构建的进化树显示：番茄的tetraspanin3基因与葡萄同源基因在一个独立的进化分支上.tetraspanin3基因在各器官中均有表达,在茎、老叶、花中...

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Published in南京农业大学学报 Vol. 38; no. 6; pp. 896 - 900
Main Author 裴延飞 刘廷利 连梓伊 杨郁文 陈天子 王金彦 张保龙 柳李旺
Format Journal Article
LanguageChinese
Published 2015
Subjects
功能分析
番茄黄化曲叶病毒
病毒诱导的转基因沉默
跨膜蛋白
Online AccessGet full text
ISSN1000-2030

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Summary:[目的]本文旨在研究tetraspanin3基因在抗番茄黄化曲叶病毒（TYLCV）病中的作用及对番茄生长发育的影响.[方法]通过MEG 5.0软件构建进化树,分析tetraspanin3基因的进化;采用实时荧光定量PCR（RT-qPCR）检测tetraspanin3在番茄各个器官中的表达量及对TYLCV诱导的响应;通过病毒诱导基因沉默技术（VIGS）将番茄中的tetraspanin3沉默,测定基因沉默后番茄的抗病性并观测沉默植株表型.[结果]构建的进化树显示：番茄的tetraspanin3基因与葡萄同源基因在一个独立的进化分支上.tetraspanin3基因在各器官中均有表达,在茎、老叶、花中表达量较高,在根、果实中表达量较低,在新叶中的表达量处于中间水平.tetraspanin3受TYLCV诱导后上调表达.沉默tetraspanin3后的植株出现了叶片扭曲,并且在接种TYLCV后,TYLCV病毒量降低50%以上.[结论]tetraspanin3与番茄叶片的生长发育有关,并且对TYLCV侵染番茄植株起正调控作用.
Bibliography:PEI Yanfei,LIU Tingli,LIAN Ziyi,YANG Yuwen,CHEN Tianzi, WANG Jinyan,ZHANG Baolong, LIU Liwang（ 1.State Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboraory of Horticuhural Crop Biology and Genetic Improvement （ East China）, Ministry of Agriculture/College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China ; 2.Provincial Key Laboratory of Agrobiology ,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China）
[Objectives]The research was conducted to study the effect of tetraspanin3 on the resisitance to Tomato yellow leaf curl virus（TYLCV）and development of tomato. [Methods]The phylogenetic tree of tetranspnin3 was constructed by the MEG 5.0 software to analyse the evolution of tetraspanin3;the expression pattern of tetraspanin3 in different organs and the response of tetraspanin3 during TYLCV infection were determined by RT-qPCR;the virus induced gene silencing（VIGS）technology was used to silence the tetraspanin3 gene and the resistance to TYLCV was tested in silenc
ISSN:1000-2030