中缅树鼩三个多潜能基因cDNA片段的克隆和序列分析

该实验从中缅树鼩（Tupaia belangeri）组织中成功克隆Klf4、Sox2和c-Myc基因的部分序列,长度分别为382、612和485bp,分别编码127、204和161个氨基酸。树鼩的Klf4、Sox2和c-Myc序列与人相应序列的相似性分别为89%、98%和89%。将树鼩Klf4、Sox2和c-Myc基因分别与其他物种的这3个基因进行系统进化分析,发现Klf4和Sox2基因所构建的系统进化树结构较为一致,但与c-Myc所构建的系统进化树有所不同,表明这些基因在进化上存在差异。Klf4、Sox2和c-Myc基因的克隆为进一步研究其功能奠定了基础。...

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Bibliographic Details
Published in动物学研究 Vol. 34; no. 2; pp. 127 - 131
Main Author 王彩云 马云瀚 何大健 杨世华
Format Journal Article
LanguageChinese
Published 2013
Subjects
c-Myc
Klf4
Sox2
序列分析
树鼩
Online AccessGet full text
ISSN0254-5853

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Summary:该实验从中缅树鼩（Tupaia belangeri）组织中成功克隆Klf4、Sox2和c-Myc基因的部分序列,长度分别为382、612和485bp,分别编码127、204和161个氨基酸。树鼩的Klf4、Sox2和c-Myc序列与人相应序列的相似性分别为89%、98%和89%。将树鼩Klf4、Sox2和c-Myc基因分别与其他物种的这3个基因进行系统进化分析,发现Klf4和Sox2基因所构建的系统进化树结构较为一致,但与c-Myc所构建的系统进化树有所不同,表明这些基因在进化上存在差异。Klf4、Sox2和c-Myc基因的克隆为进一步研究其功能奠定了基础。
Bibliography:53-1040/Q
Tree shrew; Klf4; Sox2; c-Myc; Sequence analysis
In this paper, partial sequences of the tree shrew （Tupaia belangeri） Klf4, Sox2, and c-Myc genes were cloned and sequenced, which were 382, 612, and 485 bp in length and encoded 127, 204, and 161 amino acids, respectively. Whereas, their eDNA sequence identities with those of human were 89%, 98%, and 89%, respectively. Their phylogenetic tree results indicated different topologies and suggested individual evolutional pathways. These results can facilitate further functional studies.
Cai-Yun WANG , Yun-Han MA , Da-Jian HE , Shi-Hua YANG （1. Faculty of Life Science and Technology, Kunming 650500, China,＂ 2. Faculty of Medical, Kunming University of Science and Technology, Yunnan Kunming 650500, China,＂ 3. Kunming lnstimte of Zoology, the Chinese Academy of Sciences, Yunnan Kunming 650223, China）
ISSN:0254-5853