草甘膦降解菌A-F02的分离·鉴定及降解特性研究

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Bibliographic Details
Published in植物病虫害研究：英文版 Vol. 1; no. 5; pp. 54 - 57
Main Author 吴学华 付桂明 万茵 郭德斌 陈勇辉 罗阳帆 吴晓芳
Format Journal Article
LanguageEnglish
Published 2010
Subjects
生物量
生物降解率
米曲霉A-F02
草甘膦
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ISSN2152-3932

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Bibliography:WU Xue-hua1,2,4,FU Gui-ming1,2,3,WAN Yin1,3,GUO De-bin1,2,4,CHEN Yong-hui1,2,4,LUO Yang-fan1,2,3,WU Xiao-fang1,2,4 1.State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China;2.Sino-German Food Engineering Center,Nanchang University,Nanchang 330047,China;3.Food Engineering Department of Life Science and Food Engineering College,Nanchang University,Nanchang 330031,China;4.Biotechnology Deparment of Life Science and Food Engineering College,Nanchang University,Nanchang 330031,China
[Objective] The research aimed to isolate the glyphosate-degraded strain and study its degradation characteristics.[Method] A glyphosate-degraded fungal strain A-F02 was isolated from sludge in an aeration tank of a glyphosate manufacture.The fungal strain A-F02 was identified according to morphological characteristics and internal transcribed spacer（ITS）region of nuclear ribosomal DNA sequence analysis.The glyphosate-biodegraded characteristics of strain A-F02 and the influencing factors were studied.[Result] The fungal strain A-F02 was identified as Aspergillus oryzae sp..The glyphosate-biodegraded rate was 86.82% in the mineral salt medium with 1 000 mg/L of glyphosate as the sole source of carbon,after being incubated at 30 ℃ and 150 rpm for 7 d.The biodegradation rates and biomass of the A-F02 were the highest under the culture conditions with glucose（0.5%,w/v）,pH 7.5,30 ℃ and glyphosate（1 500 mg/L）.[Conclusion] The research provided the experimental basis for glyphosate-biodegraded enzyme purification.
lyphosate-biodegraded rate; Biomass; Aspergillus oryzae sp.; A-F02
ISSN:2152-3932