（18）F-FDG PET/CT显像对心肌“缺血记忆”的分子机制研究

建立了不同程度的犬急性心肌缺血-再灌注模型。利用18 F-FDG PET/CT动态心肌显像和实时定量PCR方法探讨了缺血心肌葡萄糖代谢改变（缺血记忆）和缺血程度的关系。将8只杂种犬随机分为球囊封堵20min组（4只）和40min组（4只）,在空腹状态下（禁食〉12h）行基础、缺血1h和缺血24h的18 F-FDGPET/CT动态心肌显像以及99 Tcm-MIBI SPECT心肌灌注显像。计算缺血区和非缺血区心肌的葡萄糖摄取率比值K。所有显像完成后处死实验犬并分别取缺血区、非缺血区心肌组织。应用实时定量PCR方法,测定葡萄糖转运体蛋白1、4（GLUT-1、GLUT-4）、心脏脂肪酸结合蛋白（H-...

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Published in同位素 Vol. 25; no. 1; pp. 47 - 52
Main Author 谢博洽 杨敏福 叶珏 杨子鹤 窦克非 韩春雷 田毅
Format Journal Article
LanguageChinese
Published 2012
Subjects
18F-FDG
PET/CT
心肌缺血
缺血记忆
葡萄糖代谢
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ISSN1000-7512

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Summary:建立了不同程度的犬急性心肌缺血-再灌注模型。利用18 F-FDG PET/CT动态心肌显像和实时定量PCR方法探讨了缺血心肌葡萄糖代谢改变（缺血记忆）和缺血程度的关系。将8只杂种犬随机分为球囊封堵20min组（4只）和40min组（4只）,在空腹状态下（禁食〉12h）行基础、缺血1h和缺血24h的18 F-FDGPET/CT动态心肌显像以及99 Tcm-MIBI SPECT心肌灌注显像。计算缺血区和非缺血区心肌的葡萄糖摄取率比值K。所有显像完成后处死实验犬并分别取缺血区、非缺血区心肌组织。应用实时定量PCR方法,测定葡萄糖转运体蛋白1、4（GLUT-1、GLUT-4）、心脏脂肪酸结合蛋白（H-FABP）基因的mRNA表达量。两组犬三次心肌灌注显像均未见异常。基础显像时,两组预封堵心肌与非缺血区心肌18 F-FDG摄取率的比值K无明显差异;缺血1h后,两组K值均增加,且40min组高于20min组;缺血24h后,40min组的K值仍高于基础状态,而20min组的K值与基础状态下无明显差异。PCR结果示,两组非缺血心肌的GLUT-1、GLUT-4以及H-FABP基因mRNA表达量无明显差异,而缺血心肌的GLUT-1和GLUT-4基因mRNA表达量增加,H-FABP基因的mRNA表达量减低,且40min组的改变较20min组更为明显。以上结果提示,心肌缺血-再灌注后,缺血心肌对葡萄糖（18 F-FDG）摄取增加的程度和持续时间（缺血记忆）与缺血的程度相关。
Bibliography:11-2566/TL
This study was aimed to explore whether the changes of mRNA and the existence and duration of ischemic 18F-FDG uptake correlate with the extent of myocardial ischemia in ischemia-reperfusion canine model.The 20-minute（n= 4） and 40-minute（n= 4） coronary artery occlusion followed by 24 h of open-artery reperfusion in canine model were performed.All dogs underwent fasting（12 h） dynamic 18F-FDG PET/CT and 99Tcm-MIBI SPECT imaging at baseline,1 h and 24 h after reperfusion.When all imaging were completed,myocardial samples from the ischemic and nonischemic region were obtained,and the mRNA expression of glucose transporter-1（GLUT-1）,glucose transporter-4（GLUT-4）,and heart-fatty acid binding protein（H-FABP） were estimated by Real Time PCR.There was no difference in the ratio of hypoperfused region/nomoperfused region of 18F-FDG uptake between the 20-minute group and 40-minute group at baseline.When examined at 1 h,increased 18F-FDG uptake was observed in the 40-minute group.When estimated at 24 h,only the
ISSN:1000-7512