家兔脑型血吸虫病动物实验模型的建立及影响因素
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| Published in | 神经科学通报：英文版 Vol. 27; no. 2; pp. 91 - 98 |
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| Main Author | |
| Format | Journal Article |
| Language | English |
| Published |
2011
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1673-7067 1995-8218 |
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| Bibliography: | experimental model; schistosomiasis; neuroschistosomiasis; granuloma Objective The present study aimed to establish a cerebral schistosomiasis model in rabbits,to provide a valuable tool for morphological analysis,clinical manifestation observation,as well as investigations into immunological reactions and pathogenesis of focal inflammatory reaction in neuroschistosomiasis（NS）.Methods Sixty New Zealand rabbits were randomly assigned into operation,sham-operation and normal groups.Rabbits in the operation group received direct injection of dead schistosome eggs into the brain,while their counterparts in the sham-operation group received saline injection.Rabbits in the normal group received no treatment.Base on the clinical manifestations,rabbits were sacrificed on days 3,5,7,10,20,and 30 post injection,and brain samples were sectioned and stained with hematoxylin-eosin.Sections were observed under the microscope.Results The rabbits in the operation group exhibited various neurological symptoms,including anorexy,partial and general seizures,and paralysis.The morphological analysis showed several schistosome eggs in the nervous tissue on day 3 post operation,with very mild inflammation.On days 7-10 post operation,several schistosome eggs were localized in proximity to red blood cells with many neutrophilic granulocytes and eosinophilic granulocytes around them.The schistosome eggs developed into the productive granuloma stage on days 14-20 post operation.On day 30,the schistosome eggs were found to be in the healing-by-fibrosis stage,and the granuloma area was replaced by fibrillary glia through astrocytosis.The sham-operation group and the normal group showed negative results.Conclusion This method might be used to establish the cerebral schistosomiasis experimental model.Several factors need to be considered in establishing this model,such as the antigenic property of eggs,the time of scarification,and the clinical manifestations. 31-1975/R Peng WANG,Dan WANG,Shi-Jie CHEN,Ming-Can WU,Xiang-Lin CHENG,Jun-Chuan LI,Ting-Xuan CHEN,Zhan-Sheng ZHU 1Department of Neurosurgery,2Department of Central Laboratory,3Department of Neurology,4Department of Pathology,the First Affiliated Hospital,Yangtze University Clinic Medical College,Jingzhou 434000,China Department of Neurosurgery,Wuxi No.2 People＇s Hospital,Wuxi 214002,China |
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| ISSN: | 1673-7067 1995-8218 |